| Pickering emulsion can be used as a carrier for drug delivery on human skin.It can not only avoid the damage of traditional surfactants to the skin,but also benefit to the topical delivery of drugs.Therefore,it is very promising to use Pickering emulsion in the transdermal delivery of actives in cosmetics.This paper used starch nanocrystals(SNC)as the base particles to prepare octenyl succinic anhydride modified starch nanocrystals(OSA-SNC)and ?-polylysine complexed starch nanocrystals(?-GPL/SNC).They were used to prepare Pickering emulsions containing the Coenzyme Q10.These emulsions were embedded in transdermal transportation and the mechanism of transdermal transportation was explored.It provides the foundation for the application of this kind of carrier in transdermal delivery.Firstly,the Pickering emulsion stablized by SNC was used to carry the Coenzyme Q10.And the in vitro transdermal delivery of actives was tested by Franz diffusion cell method.In order to establish the best transdermal model of coenzyme Q10,the single factor experiment was conduct.The factors such as stratification of pigskin,different receiving solution,different extractants,different extraction methods and different extraction times that affect the content of coenzyme Q10 in pig skin were explored.The experimental results showed that when pig skins were extracted by layers the maximum content of coenzyme Q10 was obtained.And the PBS(pH=7.4)was chosen as the receiving liquid.The isopropanol was chosen as the extractant.The sonication of samples for 30 min and extracted for one time to achive the maximum content of coenzyme Q10.Secondly,to obtain octenylsuccinic anhydride modified starch nanocrystals(OSA-SNC)with different degrees of substitution,octenylsuccinic anhydride was used to hydrophobically modify SNC.Then the effect of modification on the emulsification properties of SNC was studied.The results showed that the Pickering emulsion stablized by OSA-SNC with low degree of substitution had good performance.For the transdermal delivery experiment,the Pickering emulsion carrred with coenzyme Q10 obtained higher transdermal content of coenzyme Q10 compared with the coenzyme Q10 oil solution.The penetrating position in pig skin and the changes of the stratum corneum of pig skin were analysed,which were used to investigate the the effect of OSA modification on transdermal transportation of coenzyme Q10 in the skin.The results showed that OSA modification can not increase the transdermal content of coenzyme Q10.Finally,in order to increase the penetration of Coenzyme Q10 in the skin,?-GPL was chosen as a penetration enhancer.The effects of concentrations of ?-GPL on properties of ?-GPL/SNC particles were investigated.And the emulsification of the mixed particles was verified by the storage stability of the emulsions.The results showed that with the increase of the concentration of ?-GPL,the stability of the emulsions increased first and then decreased.The emulsion was most stable when the mass fraction of ?-GPL was 0.2% of the aqueous phase.?-GPL/SNC stabled coenzyme Q10-embedded Pickering emulsion,SNC stabled coenzyme Q10-embedded Pickering emulsion,and coenzyme Q10 oil solution were used in transdermal experiment.The transdermal content and the position of coenzyme Q10 of pig skin were compared.The changes of the structure of the stratum corneum of pig skin were analyzed,which not only was used to study the effect of ?-GPL as a penetration enhancer on the transdermal transport of coenzyme Q10 in the skin,but also was used to explore the transdermal mechanism of its stable Pickering emulsion.The results showed that the Pickering emulsion as a carrier is conducive to the penetration of coenzyme Q10 in the skin,and using ?-GPL/SNC stabilized Pickering emulsion as a coenzyme Q10 transdermal delivery vehicle could enhance the transdermal delivery of Coenzyme Q10 in the skin. |
PDF Full Text Request