| Semiconductor ZnO nanoparticles exhibited high chemical stability,electrochemical activity,biocompatibility,and rapid electron transfer kinetics,and was widely used in biomedical field.As novel two-dimensional materials,molybdenum disulfide and black phosphorus?BP?are indirect bandgap materials,which are widely used in electrical and optical applications.When molybdenum disulfide and black phosphorus are incorporated with other nanomaterials,the performances of nanocomposites were often improved,which could further expand their applications.In this thesis,electrogenerated chemiluminescence?ECL?of ZnO nanorods?ZnONRs?,BP quantum dots doped ZnO nanoparticles?BPQDs@ZnO?,ZnO immobilized MoS2 nanosheets?ZnO/MoS2?were investigated,and several ECL sensors were fabricated.The main research contents are as follows:1.In the present work,ZnO nanorods?ZnONRs?were synthesized by hydrothermal method,and characterized by field emission scanning electron microscopy?FE-SEM?and X-ray diffraction?XRD?.ECL behaviors of ZnONRs were investigated in neutral aqueous condition in the presence of K2S2O8.Cyclic voltammetry?CV?results revealed that ZnONRs can react with K2S2O8 to generate strong light emission,revealing that K2S2O8can act as coreactant of ZnONRs ECL.ZnONRs synthesized under different pH conditions exhibited different ECL intensities,and the most intense ECL signal was obtained at pH7.0.Cytochrome C could compete with ZnONRs to react with K2S2O8,and exhibited apparent inhibiting effect on ZnONRs ECL,which can be sensitively detected in the range of 0.01 nM to 5 nM,with a detection limit of 4.7 pM?3??.The present ECL system exhibited high sensitivity and good stability,which is suitable for the fabrication of novel ECL sensors.2.In the present work,black phosphorus quantum dots?BPQDs?were synthesized by liquid exfoliation method,which was added into the precursor solution of ZnO nanorods to synthesize BPQDs doped ZnO nanoparticles?BPQDs@ZnO?by hydrothermol method.The doping of BPQDs could inhibit the further growth of ZnO nanorods,resulting in the formation of cubic BPQDs@ZnO nanoparticles.The obtained BPQDs@ZnO nanoparticles were characterized by field emission scanning electron microscopy,transmission electron microscopy,X-ray diffraction,X-ray photoelectron spectroscopy,and Raman spectroscopy,III respectively.Electrogenerated chemiluminescence?ECL?of BPQDs@ZnO was investigated under neutral condition.Cathodic ECL signal was obtained in the presence of K2S2O8,which is stronger than that of ZnO nanorods.Cytochrome C exhibited apparent inhibiting effect on BPQDs@ZnO ECL,and could be sensitively detected in the range of1.0×10-99 to 5.0×10-77 mol/L with a detection limit of 0.96 nM?3??.The obtained results revealed the potential ECL behaviors of BPQDs involved nanocomposites,which is promising in biosensing application.3.ZnO doped MoS2 nanocomposites?ZnO/MoS2?were synthesized by hydrothermal method,and characterized by scanning electron microscopy,transmission electron microscopy,X-ray diffraction,X-ray photoelectron spectroscopy,and Raman spectroscopy,respectively.The morphology of ZnO nanoparticles changed from rod-like to cubic shape after it was doped with MoS2 nanosheets,revealing that the MoS2 layers could limit the growth of ZnO alongc-axis.Electrogenerated chemiluminescence?ECL?of ZnO/MoS2nanocomposites was investigated in neutral condition with K2S2O8 as coreactant,and strong cathodic ECL signal was obtained,which is stronger than that of pure ZnO nanomaterials.Cysteine exhibited apparent inhibiting effect on ECL signal,and could be sensitively detected.In the range of 1.0×10-88 to 1.0×10-66 mol/L,the decreased ECL intensities varied linear with the logarithm of cysteine concentration,and the detection limit was calculated as 6.5×10-9mol/L?3??.The proposed ECL sensor exhibited high sensitivity and good stability,and revealed the potential application of 2D nanomaterials in ECL sensing field. |
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