Controllable Preparation Nanotheranostic Particle For Dual-modality Imaging Guided Photothermal Therapy

A multifunctional diagnosis reagent was prepared by combing two or more kinds of materials with photothermal thermal reagent which can be effectively diagnosis and treat of the lesion site.The multifunctional imaging guided photothermal therapy(PTT)reagent exhibits great potential for diagnosis and treatment.Lead sulfide(PbS),as a high atomic number material which have a lot of advantages such as admirable X-ray absorption and higher photothermal conversion efficiency.It is very meaningful to prepare theranostic agent which combined with high organization penetration performance magnetic resonance(MR)imaging.Fluoresence imaging has been widely applied to diagnose combined with X-ray computer tomography(CT)image,due to the advantages of high sensitivity,high spatial resolution and high penetrability.It is very necessary to produce CT/fluoresence imaging guided theranostic agent regent which combined with PTT.In this paper,we design and fabricate a relative simple and controllable approach for the preparation of gadolinium(III)complexes-grafted-lead sulfide(GCGLS)nanoparticles or high-performance CT/fluoresence imaging guided photothermal therapy by ATRP to achieve a theranostic nanoparticle for combined CT and MR imaging or CT and fluoresence imaging guided PTT.The specific process is as follows:In this work,we report a controllable method(Atom Transfer Radical Polymerization(ATRP))for the preparation of gadolinium(?)comp lexes-grafted-lead sulfide(GCGLS)nanoparticles.And a series of characterizations(such as TEM,HR-TEM,EDX,XRD,FTIR,etc.)proves that the particle size of GCGLS nanoparticles is about 11 nm By control the addition amount of gadolinium(III)complexes,the sizes of GCGLS have no evident change.The tenperature of GCGLS nanoparticles solutions can quickly elevate to 57.5? in 10 min under laser irradiation(808 nm,1.5 W/cm2)and reveal a higher photothermal conversion efficiency(?31%).When the GCGLS nanoparticles are injected into the mice,the tumor site is clearly observed efficient accumulation.Moreover,the nanoparticles has excellent a prominent X-ray computer tomography(CT)and T1-weight magnetic resonance(MR)imagings in vitro and in vivo.By combination of GCGLS and near-infrared laser irradiation,the effective tumor treatment experiment is conducted achieving in mice.Therefore,the prepared GCGLS nanoparticles with dual-modality imaging guided PTT can be used as a potential diagnosis and treatment reagents for clinical applications.Herein,we prepared oxidized dopamine(OD)nanoparticle which catalyzed by copper,then the europium(III)complexes which involved CT and fluorescence properties(Eu(AA)2(DTA)Phen)were grafted to the surface of OD to synthesise europium complexes-grafted-oxidized dopamine(ECOD)nanoparticles by ATRP,and achieve a theranostic agent for combined high-performance CT and fluoresence imaging guided PTT.It has demonstrated many excellent characteristics:the ECOD nanoparticles have excellent water dispersibility and high iodine content;the nanoparticles have excellent a prominent effective of CT and fluoresence imagings by a test of images in vitro and in vivo;the ECOD not only reveal a higher photothermal efficiency and photothermal conversion efficiency when near-infrared laser irradiate 10 min at 808 nm,but also shows an obvious photothermal therapy efficiency in tumors site after injected into the mice.Experimental results show the high-performance CT/fluoresence imagings guided photothermal therapy reagents have a potential research prospect in biomedical field.