The Synthesis And Application Of Fluorescent Probes For Endogenous Enzyme And Gasotransmitters

Fluorescent probes with high sensitivity,high selectivity and good biological compatibility are widely used in chemistry,materials chemistry,environmental science and biomedical fields.In this paper,four kinds of fluorescent probes have been designed and synthesized for quantitative detection and bioimaging of endogenous enzyme and gasotransmitter.These probes can detect alkaline phosphatise,carbon monoxide,nitric oxide and hydrogen sulfide,respectively.Details are as follows:Firstly,a hemicyanine-based near-infrared(NIR)fluorescent probe named CyP is first synthesized and used to detect ALP activity.The probe possesses a facile structure,which can be obtained by easy synthetic steps.Besides,the fluorescence emission of the sensing system is at 738 nm belonging to NIR region,which is suitable for bioimaging in vivo.The probe exhibits high sensitivity to ALP with 10-fold fluorescence enhancement and low detection limit(0.003 U/mL)can match the level of ALP in vivo.Furthermore,the NIR fluorescent probe is applied for the detection of endogenous ALP activity in various biological samples such as cell,tissue with satisfactory results.Secondly,a hemicyanine-based near-infrared(NIR)fluorescent probe named CyAPC is first synthesized and used to detect mitochondrial CO.The fluorescence emission of the sensing system is at 736 nm belonging to NIR region,which is suitable for bioimaging in vivo.Besides,CyAPC,a positively charged molecule,would have a high tendency to localize in mitochondria of cells.As far as we know,CyAPC is the first NIR fluorescent probe to detect exogenous and endogenous CO in mitochondrion.Furthermore,the NIR fluorescent probe is applied for the detection of exogenous and endogenous CO in various biological samples such as cell,tissue with satisfactory results.Thirdly,an ER-targeted fluorescent probe named ER-Nap-NO for imaging of NO is disclosed.The probe displays several obvious advantages including high sensitivity,selectivity and ER-targetable ability.Based on these excellent properties,the probe is applied for the two-photon imaging of the exogenous and endogenous NO in ER of living cells.Finally,a novel dual-detection fluorescent probe named Mito-VS for imaging of H2S and viscosity changes in mitochondria is disclosed.The probe could provide high sensitivity and selectivity toward H2S and viscosity.Finally,Mito-VS was successfully applied to imaging the fluctuation of mitochondria viscosity and H2S in HeLa cells.