Study On The Technological Conditions Of Tagatose Fermentation

The molecular formula of D-tagatose is C₆H₁₂O₆,with a molecular weight of 180.1661.D-tagatose is one of a new type of functional rare s?gar.D-tagatose has many benefits for the human body,such as low blood s?gar,anti-dental caries,control obesity,and improved intestinal beneficial flora.D-tagatose can be added to foods,beverages,functional s?gar health products and other products as a food additive.It is an ideal glycogen for people with diabetes,high blood s?gar and obesity,and can replace traditional high-energy s?gars such as sucrose,glucose,and maltose.At present,the industrial production of D-tagatose is mainly chemical synthesis.However,chemical synthesis also has some disadvantages,such as under the alkaline conditions and the catalysts action of metal ions will produce many Chemical pollutants and byproducts.The production and purification process is difficult.There are also issues such as high energy consumption and pollution.Biotransformation is a process that uses cell enzymes as a catalyst to convert D-galactose or galactitol to D-tagatose.In this project,we used biotransformation to produce D-tagatose.The strain we use is Lactobacillus,a safe food producing bacterium and an essential probiotic for the human body.We mainly used microbial fermentation technology to solve the problems of high cost,low conversion rate,environmental protection and food safety in the production process.The main results of this experiment design are as follows:1)Determine the analytical method of D-tagatose:Thin layer chromatography,resorcinol spectrophotometry and HPLC.2)The optimal culture formula for D-tagatose production strains was determined:0.5%L-arabinose,1%yeast powder,1%peptone,1%sodium acetate,0.02%potassium dihydrogen phosphate,0.005%magnesium sulfate,200?g/L thiamine,200?g/L biotin,0.02%calcium chloride,0.002%zinc sulfate,0.008%manganese sulfate,pH 6.5-7.0.3)Optimization of reaction conditions and experimental exploration to increase the conversion of D-tagatose;The reaction conditions were optimized:The reaction volume is 10 mL,the reaction temperature is 37°C,and the reaction time is 32-34 h.The result of experimental:The conversion of D-tagatose increased to 46.21%with 1g of CaCO3 as a buffer and 0.9%of L-arabinose.