Preparation And Biomimetic Mineralization Application Of Peptide,Silk Protein And Graphene Three-dimensional Hybrid Materials

Hybrid nanomaterials with tailored functions,consisting of self-assembled peptides and silk fibroin,have been intensively applied in nanotechnology,tissue engineering,and biomedical applications due to their unique structures and properties.Herein,a peptide and silk protein-mediated biomimetic strategy is adopted to create the multifunctional three-dimensional(3D)graphene-based hybrid minerals.In this work,one nanohybrid material was prepared on the basis of 3D GF and peptide nanosheets and another nanohybrid material was based on 3D graphene oxide and silk protein nanoparticle.The characteristics and morphology analysis of self-assembly of peptides and silk protein,the porosity of three-dimensional graphene foam,and the application of hybrid materials in biomimetic mineralization were studied.The main contents and conclusions are as follows:1.The novel peptide sequence LLVFGAKMLPHHGA was designed independently.The LLVFGAK sequence was used to promote the self-assembly of polypeptides as nanostructures.The phenylalanine benzene ring and graphene formed a ?-?conjugated structure,and the MLPHHGA sequence promoted biomimetic mineralization.Both nanoscale nanoparticles and peptide nanoscale nanostructures were prepared by regulating the reaction time,temperature and the kind of solvent,and the morphology and distribution of the products were characterized.2.Using polyurethane as a template,the graphene oxide was adsorbed in the polyurethane framework,and then the graphene oxide polyurethane material was pyrolyzed under the high temperature in nitrogen atmosphere to remove the polymer composition and obtain the three-dimensional structure of the graphene foam.Then,according to the characteristics of the new peptide nanosheets,it was adsorbed on the surface of graphene foam by ?-? conjugated accumulation force,and the three-dimensional graphene-peptide nanosheets hybrid materials were prepared successfully,and the morphology and structure were analyzed.3.In order to simulate biomimetic mineralization,we studied the formation of hydroxyapatite by three-dimensional graphene-peptide nanossheets hybrid materials.The three-dimensional graphene-peptide nanosheet-hydroxyapatite material after mineralization shows super light,porous and shape plasticity.In addition,in vitro cell experiments show that the new material has good biocompatibility and is beneficial to cell adhesion.4.The graphene oxide,which were activated by EDC/NHS,were combined with the silk protein nanoparticles extracted from silkworm cocoons.Then the graphene oxide silk protein composite membrane was prepared by layer layer vacuum filtration,and the silk protein nanoparticles were fully utilized to promote the nucleation and growth of hydroxyapatite,and the graphene oxide-silk protein composite membrane is used in biomimetic mineralization.