Research On The Preparation And Application Of Functionalized Magnetic Graphene Nano Drug Carriers

Chemotherapy is one of the most promising treatments for most types of cancer.However,the current treatment methods primarily rely on conventional drugs that have only limited effectiveness and serious side effects.Thus,novel target-specific drug carriers and synergistic therapies have become a hot topic of research.In this thesis,two novel multifunctional nano drug carriers were constructed by using Fe₃O₄ nanoparticles and supramolecular polymers and applied to the delivery of anticancer drugs.These new multifunctional nanomaterials with superior superparamagnetism,good biocompatibility,significantly high photothermal conversion and cancer targeting can be used as multi-targeted chemical-photothermal synergistic drug carriers.It provides a new strategy for the synthesis and application of new nano-drug carriers.The main chapters are summarized as follows:Chapter 1:The superior,preparation,functionalization,research progress and applications in biomedicine of graphene-based drug nanocarriers have been emphatically discussed in this chapter.Chapter 2:A novel multifunctional?-cyclodextrin-hyaluronic acid polymer modified magnetic graphene oxide nanomaterial?MGO@CD-HA?was constructed successfully.The morphology,particle size,magnetic properties and specific surface area were characterized by TEM,XRD,VSM and BET analysis.The results show that the MGO@CD-HA has exhibited large specific surface area,superior superparamagnetism and good dispersion in water.The high photothermal conversion rate of MGO@CD-CA-HA has been confirmed by irradiating with near infrared light,which lays a foundation for the application of the treatment of photothermal therapy.Chapter 3:The drug carrying ability of MGO@CD-HA has been studied by using doxorubicin hydrochloride?DOX?as a model drug.The loading and release behaviors were investigated by fluorescence spectroscopy.Besides,the fluorescence images and MTT assays of human hepatoma cell BEL-7402further showed that the drug carrier had chemical-photothermal synergistic effect,which indicated that the multi-functionalized MGO@CD-HA was an efficient drug carrier with targeted delivery and controlled release for chemical-photothermal synergistic therapy.Chapter 4:The?-CD-cholic acid-hyaluronic acid polymer??-CD-CA-HA?was synthesized by grafted cholic acid and?-CD onto natural hyaluronic acid.The MGO@CD-CA-HA was prepared by anchoring?-CD-CA-HA on the surface of nano magnetic graphene oxide.The properties of MGO@CD-CA-HA have been characterized detailly and indicate that it has large specific surface area,good dispersion and superior superparamagnetic properties.In addition,the high photothermal conversion rate of MGO@CD-CA-HA has been confirmed by irradiating with near infrared light,which can be used as a new type of photothermal conversion material.Chapter 5:The hydrophobic anticancer drug camptothecin?CPT?as a model drug was loaded onto the MGO@CD-CA-HA.The drug loading and the release performance under different pH and NIR irradiation were investigated,and the maximum drug loading of CPT is 847.4 mg·g^-1.The drug release can be promoted under periodic laser irradiation.In vitro cell experiments showed that MGO@CD-CA-HA had good biocompatibility.By comparing the inhibition and killing efficiency of MGO@CD-CA-HA/CPT on human hepatoma cell BEL-7402 and esophageal cancer cell K-150,we can see that the drug carrier has significant targeting ability for hepatoma cells,and it can enhance the efficacy of drugs when irradiated under NIR.It is indicated that MGO@CD-CA-HA performs variety of good characters as a drug nanocarrier with multiple targeting,control release,chemical and photothermal synergism.