Study On The Preparation And Cell Compatibility Of Bacterial Cellulose Composites

Bacterial cellulose(BC)is a high purity,high water holding capacity and permeable ventilation ability,adjustable physical properties and biological properties,high young's modulus and tensile strength,high biocompatibility and good biodegradability of super fine mesh structure of nanoscale fibers.The excellent performance of BC has led to a wide range of application prospects in the functional materials of medical organization,especially the good cellular affinity of BC,which can be used to fabricate flexible tissue support materials.However,due to the BC is a single fibre,and medical organization function materials need to meet a variety of requirements,the BC can be used as a bioaffinity basis material with other materials of compound preparation of all kinds of medical organization.In particular,as a research hotspot for medical stent materials,the research focus of nerve scaffold materials has attracted the attention of countless researchers,and microbial infection is an important problem to be solved in clinical practice.In this paper,firstly,the three-dimensional graphene(G)was introduced into the progress of microbial fermentation to prepare 3D graphene/bacterial cellulose(G/BC)composite.The conposite can be uesd as a scaffold cultivating neural stem cells.By chemical vapor depositon(CVD),the 3D graphene foam can be used as a skeleton structure of attached BC,and its level of micron channel can better simulate the cell growth environment,provide a good structural support,promote the adhesion,migration and proliferation of neural stem cells.The cytotoxicity test indicates that this scaffold has no obvious toxicity to cells.Thus,the materials produced have the potential to develop into neural scaffold materials.Based on the biocompatibility and good mechanical property of bacterial cellulose,graphene oxide(GO)load of silver nanoparticles(AgNPs)outer package bacteria cellulose(GO-AgNPs/BC)hydrogel micro fiber was manufactured by microfluidics.Using the GO-AgNPs as the inner structure,BC hydrogels as the outer layer,we produced several meters of composite hydrogel fibers in one minute through our homemade micro-flow cospinning device.We investigated and characterized the microfiber morphology and mechanical properties in detail.The excellent mechanical properties were proved by the micro fibers successfully being woven.The unique geometrical morphology of the micro fiber can effectively realize the sustainable release of antibacterial components.The experimental results showed that the prepared GO-AgNPs/BC composite microfibers had a better inhibitory effect on gram-positive and gram-negative bacteria in a reusable way.The cytotoxicity texts indicated that the material had small side effects on mammalian cells and could be used as a tissue friendly artificial scaffold.Therefore,the GO-AgNPs/BC hydrogel fiber provides a new idea and method for the development of biomedical antimicrobial materials.The above experiments show that,with bacterial cellulose as substrate preparation of composite material can meet different needs,suggesting that the bacterial cellulose having potential application value in medical nerve scaffold and antibacterial materials.