| Selenium is a kind of extremely important micronutrients to human health,mainly exists in the form of selenoprotein,which affects various aspects of human health such as antioxidant protection,cell redox balance,cancer prevention and other physiological and pathological processes.Due to the complex composition of biological samples and the very low selenoprotein/total protein ratio(<10-5),establishing a high sensitive selenoprotein detection method is very challenging and has very important significance to selenoproteomes research.In the present study,a new sample introduction method was developed for chemical vapor generation induced by low temperature plasma.This method offers several significant advantages for analyses,including high sampling efficiency,efficient matrix separation,avoiding chemical reagents,green and low consumption.Coupled with high sensitive atomic fluorescence spectrometer,a quantitative analysis method was proposed for As,Te,Sb and Se in aqueous solution,and successfully applied to the determination of the total content of Se in selenized yeast.To validate the accuracy of the developed method,three certified reference materials,under-water sediments,laver and kelp,were analyzed.In this paper,a new experimental equipment of solid sampling system for determination of Se was designed and manufactured based on low temperature plasma technology.Se chemical vapor generation for selenoprotein induced by hydrogen plasma generated by H2 and He discharge,and detected by atomic fluorescence spectrometer.This indirect quantitative method was successfully applied to the quantitative detection of glutathione peroxidase from bovine erythrocytes.In addition,the spatial distribution analysis of selenoprotein was accomplished in this study.Water-soluble proteins extracted from selenized yeast was separated by gel electrophoresis and then transferred to PVDF membrane,which was scanning analyzed by the solid sampling system combined with atomic fluorescence spectrometer. |
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