Properties And Application Of Electrochemical Biosensors Based On Transition Metal Zirconium Oxide Nanomaterials

In recent years,with the rapid development of nanotechnology and the continuous discovery of unique properties of nanomaterials,nanomaterials are increasingly being used to construct nanocomposite direct electrochemical sensors,including transition metal oxides and organic-inorganic nanofunctional materials.Rapid development,constantly discovering new features,has been widely used as a signal amplification tool in various biological material detection and food safety analysis and detection.In order to improve the sensitivity and selectivity of detection,the use of nanomaterials to construct electrochemical sensing has become one of the most effective methods and is a promising research field.The combination of metal oxide nanomaterials as a supported catalyst can provide large specific surface area and high catalytic activity of heteropolyacids.Electrochemical sensors based on these two materials have been studied.This article is mainly divided into the following two parts:?1?Zirconium oxide?ZrO₂?was synthesized by hydrothermal method.Nano-ZrO₂particles were used as heteropolyacid carriers for the first time.Nano-composites of ZrO₂ and heteropolyacid?NH₄?₅H₆PV₈Mo₄O₄₀?NPVMo?were modified to glassy carbon electrode?GCE?.On the surface,NPVMo/ZrO₂/GCE electrochemical sensors were prepared.The structure of the material and the morphology of the modified electrode were characterized by SEM,IR,XPS and EDX.The results showed that the nano-ZrO₂ has a large specific surface area and can support a large amount of heteropoly acids.The modified electrode was characterized by cyclic voltammetry?CV?,pulse voltammetry?DPV?and other electrochemical methods.Compared with GCE,ZrO₂/GCE and NPVMo/GCE,the electrode had high stability,rapid response,and conductivity.Good,good selectivity,high sensitivity,large surface area and other characteristics,this is due to the synergies between POM and ZrO₂ nanoparticles to provide more active sites for the modified electrode.At the same time,the electrocatalytic performances of Clenbuterol?CLB?and ractopamine?RAC?were studied by electrochemical methods such as CV and DPV.The experimental results showed that the modified electrode had good electrocatalysis for CLB and RAC.For the detection of CLB and RAC under optimal conditions,the detection limits of CLB and RAC were 5.04 nM and 93nM,respectively,and the detection range was 3-1000?M and 3-60?M.The sensor was used to detect CLB and RAC in pork samples.The recoveries were 96.2-101.1%and94.4.2-102.3%,respectively,which provided a new analytical method for the determination of lean meat extracts in biological samples.2 The nano-ZrO₂-ZnO was synthesized by co-precipitation method and characterized by SEM,IR,XPS,EDX and other methods.Zr O2-Zn O/GCE nano-ZrO₂-ZnO modified electrochemical sensor was prepared by simple drop coating method.Electrochemical methods were used to study the modified electrode.The results showed that both the epinephrine?EP?and dopamine?DA?had a pair of redox peaks on the modified electrode,which catalyzed both,and the modification was performed by pulse voltammetry.Electrodes were used for the direct determination of epinephrine and dopamine with detection limits of24 nM and 7 nM,respectively,with detection ranges of 0.5-600?M and 0.1-1300?M.The experimental results show that the modified electrode has the advantages of simple preparation,fast response and good stability.It can be used for the determination of epinephrine and dopamine in serum,and has a high application value.