Study On The Biocompatibility Of The Long-afterglow Nano Materials Of Gallate

Gallium materials,especially the near-infrared long afterglow materials of ion doped Zn₃Ga₂Ge₂O₁₀ have good chemical stability and biocompatibility,which can be applied in biomedicine.In this experiment,we propose to use Zn₃Ga₂Ge₂O₁₀ as substrate for ion doping and prepare biocompatible Zn₃Ga₂Ge₂O₁₀ with different ion doping?PLNPs?through surface modification.The research of Zn₃Ga₂Ge₂O₁₀ was prepared by sol-gel method with Zn?NO₃?₂,Ga?NO₃?₃ and Ge O2.It showed that the diameter of Zn₃Ga₂Ge₂O₁₀: Cr³⁺,Pr³⁺ was 150 nm and the sample had purity phase,the highest crystallinity and the best luminescence performance when the mole ratio of Zn?NO₃?₂ and Ga?NO₃?₃·was 4.5:2,p H was 5,firing temperature was 1000?,holding time was 3h.The excitation and emission peak located at 267 nm and 745 nm,which belonged to the range of near infrared wavelength.When Cr³⁺ was 0.01,Sm³⁺ was 0.05,the excitation and emission peak of Zn₃Ga₂Ge₂O₁₀: Cr³⁺,Sm³⁺ located at 267 nm and 745 nm.Eu³⁺,R co-doped Zn₃Ga₂Ge₂O₁₀,when Eu³⁺ was 0.05,Sr²⁺ was 0.1,the excitation and emission peak of Zn₃Ga₂Ge₂O₁₀: Eu³⁺,Sr²⁺ located at 267 nm and 745 nm.Cr³⁺,Pr³⁺,R co-doped Zn₃Ga₂Ge₂O₁₀,both,doping amounts of Cr³⁺ and Pr³⁺ were 0.01 and 0.03,Yb³⁺ was 0.3,Zn₃Ga₂Ge₂O₁₀: Cr³⁺,Pr³⁺,Yb³⁺ had the best afterglow performance.At this time,excitation and emission peak located at 267 nm and 745 nm.Surface modification of PLNPs was carried out by SiO₂ coating and polymer coating method respectively.When the calcination temperature was 950?,the suspension time of 1/3 SiO₂-PLNPs and 2/3 PEG-PLNPs was more than 48 h,and the rest is 24⁴8 h.Through the analysis of Zeta potential,infrared spectrum and transmission electron microscope,we know PEG was successfully coated and core-shell structure formed.After co-culture of PEG-PLNPs and cells,it was characterized by MTT and imaged,results showed that cytotoxicity was 1 level.Red light spots were visible in cells,indicating that PEG-PLNPs nano probes were successfully labeled in mouse osteoblasts.