Studies On Synthesis And Drug Release Of Nanofibrous Membranes Loaded Drug

Electrospining nanofibers show great flexibility in the application of drug delivery system because of large surface area and high porosity,easy operation and low production cost,which are the key points of the research.The research work of this paper is mainly divided into two parts:The first part: the sequential electrospinning method was used to produce layer-by-layer(three-layers)drug-loaded nanofibers meshes,tetracycline hydrochloride(TCH)as the model drug,and the pure collagen membranes as the surface layers,and the PLGA+TCH(poly(lactic-co-glycolic acid))as the core layer.2% glutaraldehyde(GA)was used to crosslink three-layered membranes drug-loaded 4 h.A single PLGA drug-loaded membrane was used as the control group,and three layers of drug-loaded membranes containing collagen were used as experimental groups.The structure of the three-layers drug-loaded membranes were observed by scanning electron microscopy(SEM).Fourier transform infrared spectrometer(FTIR),contact angle measuring,and differential scanning calorimeter(DSC)were used to characterize the three-layers drug-loaded membranes and uniaxial PLGA drug-loaded membrane,respectively.UV spectrophotometer were used to investigate drug release in vitro.The results showed that:(1)The three-layers drug-loaded films has three layers,the core layer(PLGA+TCH)has good spinnability,and the outer layers of collagen films has partial adhesion;(2)FTIR showed that glutaraldehyde reacted with the collagen in the three-layers drug-loaded films;(3)The three-layer drug-coated membrane modified by collagen has smaller contact angle and better hydrophilicity than the uniaxial drug-loaded membrane;(4)the heat stability of the three-layers drug-loaded films was improved;(5)the three-layers drug-loaded films release period than the single PLGA drug-loaded film burst release and prolonged the drug release time.The second part: on the basis of completing the preparation of three-layers drug-loaded membranes,the drug release properties of three-layers drug-loaded membranes with different diameters and thicknesses in the core layer(PLGA+TCH)were explored.Under the same conditions as other parameters,the three layers of drug-loaded membranes with different core diameters and thicknesses were prepared by adjusting the spinning voltage and spinning time of the core layer,and the drug release behavior in vitro was researched.Through in vitro drug release,it was found that the smaller the diameter of the drug were,the faster release rate were;the drug-loaded membrane thickness were thicker and the cumulative drug release rate were higher.By adjusting the diameter and thickness of the drug-loaded fiber,the rate and time of drug release can be adjusted so as to achieve sustained release and controlled release of the drug.The three-layers drug-loaded membranes can achieve sustained release and controlled release of drug,which was expected as a good drug release carrier.