Preparation And Application Of Fluorescent Probes Based On PTC Molecular

Cancer is a kind of serious disease that threat people's health.It is more important to realize the early diagnose of cancer to reduce the death rate.Fluorescence imaging has emerged as a promising approach for diagnosis of cancer for its high sensitivity,low toxicity.While most fluorescent probes involve complicated molecular design,tedious synthesis and product purification procedure,which would be limit their clinical applications.Thus,it would be more fascinating if a fluorescent probe can be developed facilely with better diagnose effect and low cost.To overcome these shortages,we have operated several experiments as follows:1.We describe a new nanorod fluorescent probe constructed by the coordinating self-assembly of copper perylenetetracarboxylate nanorods?PTC-Cu nanorods?.The morphology and structure of PTC-Cu nanorods was characterized.PTC-Cu nanorods have the size of 20×200 nm.Strong absorption and emission was observed in PTC-Cu nanorods dispersion.The MTT assay conform that PTC-Cu nanorods have good biocompatibility.PTC-Cu nanorods have perfect target ability to normal cells when PTC-Cu nanorods were cultured with cancer and normal cells.PTC-Cu nanorods have excellent target effect to normal cells in cancer and normal cells co-cultured atmosphere.When applied for four different cell lines,the same results were obtained.2.In order to distinguish normal and cancer cells more efficiency and explore the reasons why PTC-Cu nanorods select imaging normal cells,experiments were performed.Aspect ratio,surface potential,coating materials of PTC-Cu nanorods was changed to test the selectivity to normal cells.Firstly,we changed temperature,time,concentration and raw ratio of the reaction to get PTC-Cu nanorods with different aspect ratio.The results show that temperature influences the aspect ratio most.The imaging results suggest the change in aspect ratio in the present study is not sensitive to the selective cell staining behavior.Secondly,PTC-Cu nanorods were rapped with a layer of cationic polymer to change the surface charges.However,with the change of surface charges of PTC-Cu nanorods from negative to positive,the selectivity of PTC-Cu nanorods were decreased.In addition,a layer of silica was deposited onto the surface of PTC-Cu nanorods to offer a silicate surface with amidogen.Because cancer cells cannot be illumined by PTC-Cu@SiO₂-NH₂ nanorods directly,Rhodamine B was introduced to our system.The high imaging contrast between the cancer and normal cells makes it possible to see the statues of the normal cells among the cancerous ones.3.Magnetic fluorescent probe have attracted much more attention for its fluorescent imaging,MRI,and magnetic separation ability.We prepared magnetic nanoclusters?MNPs?with the size of about 50 nm by solvothermal method.MNPs@PTC was prepared by simply hydrolyze PTC-APTEs on the surface of MNPs.To our great surprise,MNPs@PTC can select imaging normal cells.These make MNPs@PTC a potential fluorescent probe to distinguish and separate cancer and normal cells.With the Peptide RGD introduced into MNPs@PTC as targeting group,the selectivity of MNPs@PTC was decreased.Compared with MNPs@PTC,target group make MNPs@PTC@RGDs have selectivity to cancer cells.