Multispectral Research Of The Interaction Between Biomarker Quantum Dots And Proteins

The application of quantum dot semiconductor materials with unique optical properties in nanomaterials has attracted much attention in the field of biology,which is mainly manifested in the reverse of specific markers and fluorescence imaging.The rich protein in the organism is closely related to various biological activities and diseases,and the amino acid group on the surface of the protein can interact with various small molecular substances.The interaction between quantum dots and proteins is benefit to understand the transport and mechanism of quantum dots in the body.At the same time,discuss the biological toxicity of quantum dots is great significance for the application of quantum dots in clinic is of great significance.In this paper,the oil soluble quantum dot AgInZnS is transformed into a water-soluble quantum dot AgInZnS-GO by graphene oxide which is easily soluble in water,and improves the biocompatibility of quantum dots.Human serum albumin and lysozyme as basic models,uses many kinds of spectrum technology analyze the interaction mechanism between AgInZnS-GO and proteins from many perspectives include the changes of protein in fluorescence intensity and second structure.By analyzing the homology and differentia of the interaction between AgInZnS-GO and these two proteins,establish a simple model of state complex which is produced by the combination of the quantum dot and protein.At the same time,detect cytotoxicity of quantum dots and the influence of quantum dots on cell morphology was analyzed through the above model.The specific research contents mainly include the following three aspects: first,the preparation of water-soluble quantum dot AgInZnS-GO with high biocompatibility.The particle size and distribution of quantum dots were observed by transmission electron microscopy,and the carboxyl group was distributed according to the Fourier transform infrared spectroscopy.Secondly,the effects of AgInZnS-GO quantum dot with human serum albumin and lysozyme were studied by multispectral technique.In this paper,the quenching mechanism of quantum dot and protein interaction is analyzed by fluorescence spectrometry.The ultraviolet and visible absorption spectra is to decide whether a new ground state complex was generated;The structural changes of protein and the major chemical bond changes during the reaction were analyzed by the Fourie transform infrared spectra and the circular dichromatic spectrum.Finally,analyzing and compare the spectral data,established the model of the quantum dot-protein reaction system.Thirdly,the cytotoxicity of quantum dots is analyzed.The safe concentration and time range of quantum dots were analyzed from two perspectives: cell appreciation rate and cell morphology.At the same time,combining the reaction system model analyze why the quantum dots has toxicity.The experimental results show that AgInZn-GO water soluble quantum dots have good biocompatibility,which can be combined with proteins to form new compounds and have low cytotoxicity and biological practicability.It provides an important basis for the combination of quantum dots and specific proteins and fluorescent labeling.