Study On The Inflammation Protective Effects And Mechanism Of Silkworm Pupa Oil On Digestive System

With the gradual improvement of people's living standard,disease of digestive system is also increasing.Gastric injuries,liver damage and functional constipation caused by dietary problems are the most important digestive system diseases.As a new type of oil,silkworm pupa oil is rich in unsaturated fatty acids,which is beneficial to the health of the human body.While getting a good taste,we can also recharge the essential fatty acids needed by the body to reduce the inflammation in the body and prevent the oxidation of the body at the same time.Analysis of components of chrysalis oil experiments,and by the relieving effect of silkworm pupa oil on HCl / ethanol induced gastric injury,leading to acetaminophen liver injury caused by constipation and activated carbon,reduce oxidative damage and discuss silkworm inflammation body from oxidative stress and inflammatory pathways,preliminary determination of chrysalis oil can protect digestion the efficiency of the system.(1)As a new type of edible oil,silkworm pupa oil not only has good taste,but also has good health function because of its rich unsaturated fatty acid.Silkworm pupa oil is rich in unsaturated fatty acids,which has antioxidant effect and can relieve inflammation in the body.By GC-MS analysis,there are 7 kinds of fatty acids in silkworm pupa oil,mainly are palmitic acid,oleic acid and linolenic acid.The relative content of saturated fatty acids is 30.27%,and the relative content of unsaturated fatty acids is 69.73%.The relative content of monounsaturated fatty acids(such as oleic acid)is 31.25%,and the relative content of polyunsaturated fatty acids(such as linoleic acid and linolenic acid)is 38.48%.(2)Study on the protective effect of silkworm pupa oil on gastric injury induced by HCl / ethanol in mice.It was found that silkworm pupa oil treatment could increase the content of antioxidant enzyme SOD,CAT and GSH-PX in serum,and reduce the content of cytokine IL-6,IL-12 and inflammatory factors TNF-? and IFN-?,increase the content of SST and VIP in serum and reduce the content of MTL and GT by measuring the corresponding serum indexes and some genes expression of mRNA and protein in the gastric tissue of mice.For the expression of QPCR and Western Blot,silkworm pupa oil treatment can significantly increase the content of EGF,EGFR,VEGF,eNOS,Cu/Zn-SOD,Mn-SOD and I?B-?,and reduce the content of NF-?B?Bcl-2?COX-2 and iNOS.It shows that silkworm pupa oil can protect the gastric tissue and alleviate the damage caused by HCl / ethanol on gastric tissue.(3)The protective effects of silkworm pupa oil on mice liver injury induced by acetaminophen were evaluated by determining serum index and mRNA and protein expression of some genes in liver.The experimental results show that the silkworm pupa oil was significantly reduced in mice liver cell death;reduce the liver index,kidney index and spleen index in mice;reduce the content of serum AST,ALT,TG and TC,reduce the content of cytokines IL-6 and IL-12 in serum,increased cytokine content of IL-10,increase the content of antioxidant enzymes SOD and GSH-Px.Reduce the accumulation of lipid oxidation end products of MDA,reduce the inflammatory factor TNF-? and IFN-?,decreased mRNA and Western Blot expression of NF-?B,COX-2,iNOS expression,increased expression of I?B-?.It shows that the silkworm pupa oil can protect the liver and can protect the liver from acetaminophen.(4)By measuring the daily fecal water content,intestinal propulsion rate,serum indicators Gas,MTL,ET-1,SS,AchE,SP and VIP,we made small bowel slices,and detected the expression of c-kit,SCF,iNOS and eNOS in the small intestine to study the effect of silkworm pupa oil on the prevention of constipation caused by activated carbon in mice.The results shows that silkworm pupa oil can reduce faecal water loss,promote intestinal peristalsis,increase serum Gas,MTL,ET-1,AchE,SP and VIP content,decreased the content of SS,reduce the damage to small intestinal villi by activated carbon,increase the expression of c-Kit,SCF and eNOS,reduce the expression of iNOS in small intestine.The effect of high dose silkworm pupa oil in preventing constipation is better than that of low dose group of silkworm pupa oil.Silkworm pupa oil can not only promote defecation in mice,but also reduce the damage of activated carbon to mice intestinal tract,promote intestinal self repair ability,and reduce the occurrence of intestinal inflammation.