Preparation Of Docetaxel Loaded And PFH@ICG Coated Nanoparticles Decorated With SDF-1 And The Study Of Imaging And Viability Experiments On Tongue Squamous Cell In Vitro

Objective:To prepare a Docetaxel-loaded multifunctional nanoparticles containing ICG and PFH as targeted drug delivery system,decorated with SDF-1,and characterize their general property.To evaluate drug release regular,photothermal characteristics,imaging effects,targeting ability and viability experiments on tongue squamous cell in vitro.Method:Multifunctional nanoparticles were prepared with the method of double emulsion.SDF-1 was covalently conjugated to the surface of nanoparticles through thioether bond.Their particle size,distribution and surface potential were determined by Malvern measuring instrument.The conjugation of SDF-1 was evaluated by confocal microscopy.Encapsulation efficiency,drug loading capacity and releasing regularity of nanoparticles were determined by HPLC.Photothermal property in vitro was recorded by Thermal Imager.The imaging capacity in vitro was observed by photoacoustic instrument and ultrasonic diagnostic apparatus.The targeting ability was assessed by flow cytometry.The cell activity on SCC-15 calls was checked by the CCK-8 method.Result:Targeting multifunctional nanoparticles showed regularly sphericity.The diameter was(502.88±17.92)nm.The zata potential was(-11.5±3.15)mV.The encapsulateion efficiency was 54.12%±1.74%.The drug loading capacity was 1.08 mg·mL-1.Drug releasing in vitro was initially explosiving and gradually slow.The photothermal characteristics were relating the concentration,the higher the concentration,the higher the temperature.Nanoparticles could detect significant photoacoustic and ultrasound signals.The targeting rate in vitro was 89.99%.Cell viability experiments: There were no significant difference in cell viability in SINPs groups at each concentration(P > 0.05).Compared with SINPs,the inhibition effect of DOC-SINPs appeared much stronger whether or not with NIR laser irradiation among the groups of 150?g·mL-1 and 200 ?g·mL-1(P<0.05).Conclusion:The multifunctional nanoparticle with diagnosis and treatment has been successfully prepared and has the dual-mode ultrasound / photoacoustic imaging and antitumor effects of both chemotherapy and photothermal therapy.