BODIPY-based Reactive Fluorescent Probe For Thiophenol

With the development of science and technology,reactive fluorescent molecular probes due to its high sensitivity,good selectivity,simple operation and low cost advantages have been applied to such as environmental monitoring,small molecule detection,the change of microenvironment in cells,medical diagnosis and treatment,and other fields.It has become a powerful tool for environmental monitoring and modern disease diagnosis.Aromatic thiol compounds such as thiophenol is important intermediate for pesticides,drugs and polymers which have wide ranges of industrial and agricultural application,however,high concentrations of them can cause organisms poisoning and environmental pollution.The second part of this paper plans to select BODIPY as fluorescent group,DNP(2,4-dinitrobenzene ether)as recognition and fluorescence quenching group to obtain a probe BODP,the synthesis of probe is simple and it has good fluorescence enhancement effect,under the same reaction conditions,other test compounds will not cause the result of testing.The MCF-7 cell experiments showed that the probe entered the cell rapidly with small toxicity,and had excellent intracellular imaging effect.And it can be used in the analysis and detection of thiophenol.It has good application value and prospect in chemistry,biology and environmental science.In order to further improve the properties of probe and the effect of testing,we synthesized an Off-On and long wavelength fluorescent probe BDP for selective recognition of thiophenol.The probe was synthesized by three-step reaction,and the process of synthesis was simple.The fluorescence at 585 nm after the reaction was enhanced(30 times)and the response time was fast(15 s).The detection limit was as low as 13.6 nM.Other analytes have no effect in the identification process.The cytotoxicity is low and has been successfully applied in living cells and in vivo imaging.Therefore,this work provides a quick and selective way to detect thiophenol.