A Study Of Green Synthesis Of Monolithic Column Incorporated With Nanoparticles

Graphene oxide is a kind of two-dimensional carbon nanomaterials rich in oxygen-containing groups.It has an extremely large specific surface area and can be used as a stationary phase for capillary electrochromatography because it not only provides ionized oxygen-containing functional groups Adjust the electroosmotic flow,but also to enhance the hydrophobic interaction and electrostatic interaction of aromatic polymers in the separation process.In this paper,we used methacrylic acid?MAA?and butyl methacrylate?BMA?as the functional monomer,1-hexyl-3-methylimidazoliumtetrafluoroborate?[HMIM]BF₄?andcholine chloride/1,2-propanediol as porogens,ethylene glycol dimethacrylate?EDMA?as the crosslinking agent,GO-MPS obtained by silanization of graphene oxide by?-MPS and dispersed in choline chloride/1,2-propanediol.The GO-MPS-incorporated monolithic capillary column was prepared by in-situ polymerization.The alkylbenzyl ketone analogues were separated by suitable electrochromic conditions.The maximum efficiency of the GO-MPS incorporated monolithic column reached147,000 plates/m,about twice higher than the GO-MPS-free monolith.The results show that the capillary monoliths incorporated with graphene oxide have higher separation performance than blank columns.In addition,the preparation parameters of GO-MPS incorporated capillary monolithic column were investigated,including the concentration of GO-MPS in DESs,the type of ionic liquid,the type of alcohol in DESs,the choline chloride and 1,2-propanediol in DESs,The ratio of RTIL to DESs.In addition,the orthogonal experiment design shows that the concentration of GO-MPS in DESs has the greatest impact on the overall column separation performance.The monolithic column was further applied to the separation of bovine serum albumin hydrolyzate.The results showed that it could be used for the analysis of some complex samples.The mesoporous silica coated gold nanorods have the advantages of regular pore size,large specific surface area,easy modification,high biocompatibility and physiological stability by coating mesoporous silica on the outer layer of gold nanorods.In this paper,MAA and BMA as functional monomers,1-hexyl-3-methylimidazolium tetrafluoroborate?[HMIM]BF₄?and choline chloride/ethanol as porogens,ethylene glycol dimethacrylate?EDMA?as crosslinking agent,the GNRs@mSiO₂ were dispersed in choline chloride/ethanol,and the reaction initiated by a redox initiator system.GNRs@mSiO₂ grafted monolithic capillary columns were prepared by in-situ polymerization.The results of electrochromatography on alkylbenzene analogues show that the efficiency of monolithic columns grafted with GNRs@mSiO₂?168000 plates/m?was significantly higher than the blank column?78300 plates/m?.In addition,the GNRs@mSiO₂ grafted capillary monolithic column preparation parameters were investigated,including:the concentration of GNRs@mSiO₂ in DESs,the ratio of choline chloride to ethanol in DESs,the ratio of RTIL to DESs,cross-linking degree.Through orthogonal design,the concentration of GNRs@mSiO₂ in DESs has the greatest influence on the separation performance of monolithic columns.Through the investigation of the best chromatographic performance of C1,the monolithic column has a wide range of application pH and stable performance.The GNRs@mSiO₂ doping scheme prepared organic-inorganic hybrid monolithic column has potential applications.