Preparation Of Reduced Graphene Oxide Based Aerogels And The Study On Their Adsorption For Bilirubin

Hyperbilirubinemia can cause brain damage in neonates,and can result in hepatitis,jaundice,and even death in severe cases.The current clinical treament is mainly through haemoperfusion.A successful hemoperfusion technique contains excellent adsorbent materials,which were required to have high adsorption capacity,fast adsorption rate,good blood compatibility,etc.Graphene has execellent physical and chemical properties such as large specific surface area,high mechanical properties and good biocompatibility.It can be assembled to adsorbent materials,which has high specific surface area,high strength,good biocompatibility and excellent adsorption performance.Macroporous structure will reduce the mechanical strength of graphene aerogels,however good pore structure is critical to the adsorption properties of the material.In order to get macroporous graphene sponge with high strength,different proportions of reduced graphene oxide/chitosan composites?rGO/CSn?were prepared by solution blending method.The materials were investigated via scanning electron microscope,Fourier infrared spectroscopy,X-ray diffraction,Brunauer-Emmett-Teller and electronic universal testing machines.The results showed that chitosan changed the morphology,specific surface area of the composite sponge,and greatly enhanced the mechanical strength of the composite sponge?It can withstand 8500 times its own weight?.The adsorption capacity of rGO/CS20 can reach 458.9 mg/g,which has an excellent adsorption effect.In addition,the material has good blood compatibility.The results showed that chitosan can improve the mechanical strength of composites,and graphene has excellent potential as an adsorbent for bilirubin.In order to apply the graphene material to the clinic and replace the current microspheroidal adsorption material.The reduced graphene oxide microspheres?rGOn?with high strength were prepared by Ca²⁺coagulation bath.The materials were investigated via scanning electron microscope,Fourier infrared spectroscopy,X-ray diffraction,Brunauer-Emmett-Teller and electronic universal testing machines.The results showed that rGO₈ exhibited the largest surface area of 287.5 m²·g^-1.The rGO₁₆ has the highest mechanical strength and can withstand 101,000 times its own weight.The adsorption capacity of rGO8 can reach 318.2 mg/g.In addition,rGOn had much lower hemolysis ratio and a little anticoagulant.The results showed that the reduced graphene oxide microsphere has higher mechanical strength and larger specific surface area than the reduced graphene oxide/chitosan sponge,and it has a greater application value in blood perfusion device.Finally,in order to improve the hydrophobicity of reduced graphene oxide microsphere,the reduced graphene oxide/chitosan microspheres?rGO-CSn?was formed by coating chitosan on the surface of reduced graphene oxide microspheres.The materials were characterized by scanning electron microscopy,Brunauer-Emmett-Teller and electronic universal testing machines.The results showed that rGO-CS1 exhibited the largest surface area of 252.1m²·g^-1.The rGO-CSn has the highest mechanical strength and can withstand29000 times its own weight.The adsorption performance of rGO-CSn displayed high adsorption capacity?257.9 mg/g?,faster adsorption kinetics and shorter adsorption equilibrium time?about 0.5 h?.In addition,the material has good blood compatibility.The results showed that rGO-CSn displayed a hydrophilic interface,shorter adsorption equilibrium time and good adsorption effect on bilirubin.