Preparation Of Polyelectrolyte-based Microchamber-arrays For Encapsulation And Release Of Water-soluble Small Molecules

Cargo encapsulation and controlled release are important in a large amount of areas ranging from process engineering over antibiofouling to medicine.At present,polyelectrolyte microchamber-arrays can be prepared by micro-contact printing.Polyelectrolyte microchamber-arrays have a high permeability,so it is difficult for watersoluble molecules to be effectively wrapped inside the microchambers.At present,there is less research on the release behavior of “cargo” in polyelectrolyte microchamber-arrays under different environments.For this reason,there is still much room for improvement for encapsulation and controlled release.For these deficiencies,in this thesis,polyelectrolyte-based microchamber-arrays were fabricated by layer-by-layer self-assembly technique,micro-contact printing and hot embossing.A layer of polylactic acid film is modified on the surface of polyelectrolyte microchamber-arrays,and the water-soluble reagent molecules are loaded in the mocrochambers by ultrasonic method and the polyelectrolyte microchamber-arrays are packaged,controlled release is achieved by external stimuli such as ultrasound and nearinfrared laser.Both types of release(ultrasound and laser)are also analyzed via numerical simulations.First,the polyelectrolyte multilayer film is prepared by a method of layerby-layer self-assembly using polydimethylsiloxane with a microwell array structure as a template,and a layer of polylactic acid film is modified on the surface by dipping coating.And then the polyelectrolyte multilayered polyelectrolyte film and the seal of polylactic acid are placed in solution for a period of sonication,removed from the solution and airdried naturally,and the water-soluble reagent molecules formed a precipitate in the microchambers.The polyelectrolyte microchamber-array is then transferred onto a glass sheet that has been pre-modified with a polyelectrolyte multilayer film to encapsulate it.Through ultrasonic stimulation,the structure of the microchambers are destroyed,thereby achieving the purpose of releasing the model drug.The preparation of polyelectrolyte multilayer microchamber-arrays takes a long time,and the preparation of polyelectrolyte complex microchamber-arrays is relatively easy and convenient.Using polyelectrolyte composites as raw materials,a polyelectrolyte complex microchamber-arrays can be prepared in a short time by hot embossing.Subsequently,polylactic acid is modified on its surface and the model drug rhodamine B is loaded.After the surface of the sample is modified with gold nanoparticles,the release behavior of the drug is studied by the photothermal effect through near-infrared laser irradiation.In summary,polylactic acid-modified polyelectrolyte micrchamber-arrays can effectively wrap water-soluble reagent molecules,and can effectively control the release of reagent molecules through external stimuli such as ultrasound and near-infrared laser.The polyelectrolyte microchamber-arrays,which can effectively load reagent watersoluble molecules and can be controlled release,has a good application prospect in medical implant materials.