Construction Of Polymer Micro/Nano Interface And Study On Its Compatibility With Osteoblasts

The establishment of biological interface has always been an important topic in the field of biomaterials research.It involves many fields of research,many research methods,and has important application value in real life.Although some materials in the field of medicine are biologically inert and have poor biological cell compatibility,the materials exhibit good biocompatibility after certain surface modification or surface modification of biologically active molecules.Scientists can use some surface modification methods to broad the application of materials in the biomedical field;The human cells themselves all live in a micro-nano environment,and the surface of some biomedical graft materials can be patterned,which may make the medical graft materials have an unexpected effect in the living body.The study of this topic is to start with this,there are two main parts: The first part is the preparation of two kinds of surface micro/nano structure interface materials,one is polyether ether ketone（PEEK）in situ polyaniline nanofibers,one is the surface with different micro/nano pattern of PDMS;The second part is related to the in vitro biological experiments of PDMS migration and differentiation on the surface of PDMS with different micro/nano patterns.Polyaniline nanofibers were synthesized by dilution polymerization.Polyaniline nanofibers were fabricated in-situ on PEEK sheets.In order to explore the best process parameters for the synthesis of polyaniline nanofibers,the aniline concentration and ultraviolet irradiation time were changed,and the results showed that the polyaniline nanofibers prepared under the reaction conditions of aniline concentration 7 m M UV irradiation for 30 min were the best.Then,different micro/nano patterns are designed,the designed patterns are etched on the silicon wafer by photolithography,and the micro/nano silicon pattern of the silicon wafer is modeled by soft lithography.However,the PDMS surface has a contact angle of 107-110 °.For PDMS highly hydrophobic surface and its surface and bone cells poor biocompatibility defects,we tried two surface modification methods,the final experimental results show that PDMS surface modified bioactive molecule dopamine is the best surface modification,and the modified PDMS surface has good biocompatibility with bone cells.After 8h surface modification of dopamine,the surface contact angle decreased to 70 °,and the modified PDMS surface has good biocompatibility with bone cells.Using pre-osteoblasts and chondrocytes adherent biological experiments,the results show that in the PEEK chip polyaniline nanofibers and PDMS modified dopamine two kinds of micro/nano interface material have good biocompatibility,cells adhere rapidly and grow well on both materials.PDMS-PDA with different curvatures of grooves was used to conduct osteocyte migration and differentiation experiments.At the same time,actin was detected in osteocytes in different curvature grooves.The results showed that the migration of osteocytes in grooves with different curvatures at different rates,the osteocytes migrated more rapidly in the grooves with a curvature range of 1/575-1/875μm^-1.BMSCs were cultured in the grooves with the curvature range of 1/875-1/1375μm^-1 chondrocytes induce differentiation more efficiently.Biological experiments on PDMS-PDA with different micro/nano patterns showed that bone marrow stem cells at the edge of the micro/nano pattern induced a higher rate of differentiation of chondrocytes.