Accurate Control Of Gold Nanostructure Arrangement Based On DNA Origami

DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses.In this field,nucleic acids are used as non-biological engineering materials to create static and dynamic structures.DNA origami can provide its high programmability to nanomaterials with strong addressability and flexible functionalization sites.In this case,the “bottom-up” assemble nanostructures would have novel physical and chemical properties.In this thesis,DNA origami was utilized as nanostructures carrier to fabricate a series of different gold nanoparticles(AuNPs)and gold nanorods(AuNRs)arrangement structures.The AuNPs and AuNRs arrangement structures show various surface plasmon resonance properties.The main contents of this research are described as follows:1.Designed a DNA origami template consisting of two juxtaposed ladder-like troughs and functionalized the surface sites to capture the AuNPs and AuNRs.The DNA origami-AuNP building block and DNA origami-AuNR building block were constructed by hybridizing with corresponding Au NP(11 nm)and AuNR(13×38 nm)which modified with thiol-modified DNA.2.Designed and constructed alternately arranged DNA origami-gold nanostructure building blocks.Investigated the ability of DNA origami template to regulate the arrangement of AuNPs and AuNRs.Self-assembled a series of chain-like gold nanostructure arrangement structures.3.Designed and fabricated a series of length-controllable gold nanostructures arrangement which comprise of AuNPs and AuNRs together,including homogeneous gold nanostructures arrangement and heterogeneous gold nanostructures arrangement.The ultraviolet-visible absorption spectra shown that different gold nanostructures arrangement behaved different surface plasmon resonance coupling because of the size and position.