Preparation And Properties Of PEG/PLGA Nanofiber Membranes

With the emergence of nanotechnology,the uniqueness of nanomaterials has attracted more and more attention of researchers.As a kind of electrostatic fiber manufacturing technology,electrostatic spinning is the simplest and most efficient method to produce fiber by electric field force.The prepared nanofiber membrane has the characteristics of large surface area,high porosity and easy to realize surface functionalization.The structure is similar to that of extracellular matrix and can enhance cell migration and proliferation.This technology has been widely used in many fields,such as tissue engineering scaffold,biosensor,electrospinning nanofiber membrane,biomedical field,drug carrier,wound dressing and enzyme immobilization.In this paper,polyethylene glycol(PEG)and poly(lactic acid-glycolic acid)copolymers were used as spinning materials to prepare composite nanofiber membranes by electrostatic spinning.The effects of polyethylene glycol content,spinning voltage and spinning speed on the morphology and fiber diameter distribution uniformity of composite nanofibers were discussed.The Box-Behnken experimental design and response surface surface method were used to optimize the composite nanofibers.Tensile test,contact angle test,porosity measurement and Fourier transform infrared spectroscopy(FTIR)were used to characterize and analyze the properties of the optimized fiber membrane.Furthermore,a rat model of sidewall defect-cecum abrasion was employed to investigate the efficacy of PEG/PLGA in preventing postoperative peritoneal adhesions.The results showed that through the analysis of three factors and three levels of response surface analysis,The effects of single factor on the morphology and distribution uniformity of the fibers were in the order of PEG content(A)> spinning speed(C)> spinning voltage(B).The optimum design parameters were as follows: the content of PEG was 7.5%,the spinning voltage was 19 kv,the spinning speed was 3ml / h,and the receiving was obtained.The distance is 20 cm,and the diameter of the fiber is0.82±0.254?m.Using scanning electron microscope(SEM)and Fourier transform infrared spectroscopy(FTIR)to observe the appearance of the fiber,the surface of the fiber is smooth,there is no bonding or beading phenomenon,the fiber distribution is uniform,the thickness of the single fiber is the same,the contact angle and porosity are measured,The results show that PEG can improve the porosity and hydrophilicity of PLGA nanofiber membrane.With the increase of PEG content,the contact angle of PEG / PLGA composite nanofiber membrane becomes smaller,and its hydrophilicity is improved.Adding a certain amount of PEG can improve the hydrophilicity of PEG/PLGA composite nanofiber membrane.The tensile test shows that 7.5% PEG / PLGA composite nanofiber film has good tensile strength and elongation and has better mechanical properties.In vivo applications of the PEG/PLGA on adhesion prevention indicated that the PEG/PLGA fibers were effective in reducing the formation of intraperitoneal adhesions after surgery.