Effects Of Brand's Essence Of Chicken On Regulation Of Physiology And Circadian Clock In Mice

Both the synchrony between circadian clock and environment cues and the synchrony between the master clock and peripherial clocks are critical to the maintenance of metabolic homeostasis.Studies on BRAND'S Essence of Chicken(BEC),a chicken meat extract rich in proteins,amino acids and peptides,showed its effectiveness in alleviating fatigue and promoting metabolism.Our previous study revealed that it facilitated the re-entrainment of clock genes in a rat model of circadian disruption.Here,we investigated the role of BEC in the regulation of the physiology and circadian clock using a mouse model of prolonged circadian disruption.Prolonged circadian disruption(12 weeks)resulted in hepatic maladaptation,manifested by a mild but significant(p<0.05)hepatomegaly,accompanied by disturbed hepatic lipid metabolism andliver injury.Evidently,there was marked elevations of hepatic inflammatory mediators(IL1?(11)IL6,IFN?,TNF?),suggesting an underlying inflammation leading to the hepatic injury and functional impairment.The transcription of hepatic genes involved in oxidative stress system were up-regulated by prolonged circadian disruption.Importantly,the disruption paradigm caused the alterations in the master and hepatic clock genes(Bmal1,Per1,Cry1,Dec1).The alteration might lead to the loss of circadian synchrony between the master and hepatic clock genes.Importantly,the disruption paradigm caused the decoupling between key metabolic regulators(e.g.mTOR and AMPK)and hepatic clock genes(Per1,Cry1,Dec1,Bmal1).Furthermore,we showed that the loss of circadian synchrony between the master and hepatic clock genes(Per1,Cry1,Dec1,Bmal1)could be the underlying cause of the maladaptation.When supplemented with BEC,the functional impairments were attenuated.Overall,BEC supplementation promoted the physiological adaptation to the prolonged circadian disruption through the facilitation of endogenous circadian synchrony and the coupling of circadian oscillators and metabolic regulators.