Study On Total Alkaloids Strychnineresin Composite Microcapsules Dispersd Tablets

In this experiment,total alkaloids in semen Strychni as drug model,usingcation exchange resin as drug carrier,total alkaloid drug resin complexes were prepared nux vomica alkaloids,prepared nux vomica resin compositemicrocapsules by emulsion solvent evaporation method,and is prepared intodispersible tablets,summarized as follows on the experimental content:1)To establish the U V-method for the analysis of visible spectrophotometric determination of total alkaloids strychnine,brucine absorption wavelength is 416nm,have linear equation:A=62.6C+0.0652,r=0.9973,the concentration in the range of 0.002?0.01 mg/ml;and then the 50%ethanol solution 6 times the amount of pH=5 in semen Strychni,heating reflux extraction 3 times,each time 1H;extraction recovery after ethanol,using AB-8 macroporous resin purification,drug concentration,the adsorption of 12h,distilled water 3BVelution elution,70%4BV,the flow rate was 1ml/min;total alkaloids preparednux vomica solids 1.09g,total alkaloid content of 0.768g after purification,the purity is more than 70%,effective extraction and purification method,for total alkaloids,efficient low toxicity of Strychnos nux vomica resin compoundpreparation lay the experimental foundation.2)The static exchange to prepare drug resin complexes,we investigated the effect of different factors on drug and the adsorption capacity of the resin.According to the influence of different factors on drug and resin adsorptionand ultimately determine the drug loading conditions to the final:the total alkaloids of dissolved HCl solution for 0.1 mol/L,the concentration of total alkaloids prepared nux vomica 2mg/mL solution,and the size of exchange resins at 50? reaction at the 160?180 H+ion,by differential scanningdetection of Strychnos alkaloid with ion exchange resin is through ionic bondsinstead of simple physical adsorption.3)Using F2 on drug release conditions of temperature and rotational speed ofacquaintance factor method,and the effects of the release medium on thedrug release in vitro of the resin were determined by 900ml pH6.8,the final0.1 5mol/LNaCl as release medium,in the condition of 37 C,the speed with 100r/min for the in vitro release conditions.4)By emulsion solvent evaporation method for coating on total alkaloid insemen Strychni resin compound,ethyl cellulose(EC)as capsule,adjacentbenzene two formic acid ethyl ester two(DEP)as the plasticizer,-400 of polyethylene glycol(PEG-400)as a porogenic agent in preparation of drug-resin microcapsules.Through single factor test and application of central composite design experiment,coating process and formulation optimization:EC 11.68%,DEP 11.83%,PEG-400 3.17%,in paraffin emulsion system disk:our-80:=30:3:4 of anhydrous ethanol,magnetic at 35 C,500r/min under the condition of stirring stir until no alcohol taste.5)Dispersible tablets were prepared by direct compression,the bestpreparation of dispersible tablet prescription was determined by single factor test and orthogonal test,the optimum prescription of dispersible tablets of microcrystalline cellulose as filler,cross-linked polyvinylpyrrolidone and sodium bicarbonate and citrate as the disintegrant,card combined application ofcarbomer and carboxymethyl cellulose sodium as suspending agent,talcum powder as lubricant,the prescription ratio for the 7g of microcrystalline cellulose,PVPP 0.7G,0.9g of sodium bicarbonate,citric acid 0.3g,Carbopol 0.12g,sodium carboxymethyl cellulose 0.37g,talc powder.The dispersible tablets according to "China Pharmacopoeia" relevant content were investigated,the results met the Pharmacopoeia requirements.