| In this paper,isoquercitrin-(3-glucosidase was purified from Absidia sp.R9g.The enzymatic properties were also studied,including effect of temperature,pH and mental ions on enzyme activity and enzymatic reaction kinetics.The isoquercitrin-?-glycosidase was purified from Absidia sp.R9g using Sephadex G-100 and DEAE-cellulose columns.The purity and molecular mass of the glycosidase were determined using SDS-polyacrylamide gel electrophoresis.The purified enzyme migrates as a single band on the SDS-PAGE and its molecular mass was determined to be about 50 kDa.The optimum temperature and pH of isoquercitrin-?-glucosidase was 50? and pH 5.0,respectively.This enzyme was stable at pH 3.0-7.0,below 60?.The activity of this enzyme was not obviously affected by Na+,K+,Ca2+ions,but strictly inhibited by Fe3+and Cu2+.The Vmax and Km for isoquercitrin-?-glucosidase was 3.26 mmol/L·h and 57.6 mmol/L,respectively. |
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