Organic Autoclave Preparation Of Slowly Digestible Starch Method

Slowly digestible starches(SDS),These slowly digestible starches(SDS)have a specific internal structure that delays digestion and conversion into glucose.They could be completely and d slowly digested in the small intestine,They are more slow release of energy for sustainable maintenance of postprandial glucose homeostasis to prevent the emergence of a starch insulin resistance(20~120min).As the new modified starch,SDS have the characteristics of low GI foods and slow release of energy for sustainable improvements in blood glucose loading.Diabetic human consumption may slow the rise in blood glucose to control the disease;SDS are sustainable slow release of energy to maintain a sense of fullness after eating obesity and can reduce hunger or obesity risk;SDS are sustainable slow release energy to help athletes to maintain endurance,As low GI food,SDS are visible on people to maintain normal blood glucose levels which has a positive meaning for health.SDS has a large number of foreign research and patent literature reports,some domestic research team deep research,but applied to the SDS into people's daily lives are rare on the market.This topic was mainly through optimization of three food-grade organic acids in an autoclave environment waxy maize starch acid hydrolysis conditions to achieve the purpose of improving the concentrations of SDS.it can be shown that SDS have provided a theoretical basis with a broader application in favor of the rich starch better resources development and utilization in the food processing industry.The benefits of SDS is important for the development of the food industry.The main contents of this study were based on postprandial blood glucose with a specific time variation determined a simple fast and accurate SDS in vitro determination method.Three kinds of edible organic acids(citric acid,malic acid,acetic acid)pressure and thermal acid solution of waxy maize starch to prepare SDS were optimized by statistical analysis methods(box Behnken response surface method).The process conditions of the SDS production were determined;Further optimization were purified after malic acid pressure and thermal acid hydrolysate using on the alpha-amylase to obtain higher content of SDS.At the same time,using scanning electron microscope(SEM)and differential scanning calorimetry(DSC)to SDS structure characterization and thermal characteristics were analyzed.The specific contents are as follows.1.Through the improvement of Englyst method using in vitro simulated digestion experiments refer to normal human and mouse before a meal after meal GI value,a new fast and accurate SDS in vitro detection method to determine.Detection method for 500 mg of starch samples dissolved in 20 mL of water,are added to the 15 mL pH 5.5 acetate buffer,add 10 mL mixed enzyme solution(containing porcine pancreatic alpha amylase(250 mL)and saccharifying enzyme(60U/mL)is hydrolyzed in a 37?,30 min of hydrolysis and 150 min were taken 500 ?L SDS concentration was calculated.2.The waxy corn starch as raw material,the design Expert8.0 software box Behnken design four factors and three levels,were 29 response surface experiments,respectively,to optimize the citric acid,malic acid,acetic acid,pressure and thermal acid hydrolysis conditions of preparation of SDS determined the preparation SDS process parameters to immprove the SDS concentrations.determined the process parameters were:The concentration of citric acid was 3.3%,pressure and thermal acid hydrolysis time was 37 min,pressure and thermal acid hydrolysis temperature was 115?,starch concentration 20%,SDS content was 33.06.Malic acid concentration was 2.4%,the solution temperature was 116?,the solution time was 24 min,the concentration of starch milk was 18%,and the content of SDS was 53.14%.Acetic acid concentration was 4.3%,the solution temperature was 121?,the solution time was 36 min,the concentration of starch milk was 24%,and the content of SDS was increased to 32.06%.3.Through orthogonal experiment-amylase enzyme purified malic acid obtained SDS optimization of process parameters.The amount of amylase was 250 U,reaction time was 25 min,reaction temperature was at 60?,the highest SDS concentration was 55.27%.When the 5th cycle was purified,SDS quality concentration reached 85.55%.4.scanning electron Microscope showed that SDS were purified using enzyme after malic acid hydrolysis which were making starch granule morphology changes,the appearance of the smooth to uneven,angular,debris apparently,structural changes in starch of the slowly digestible starch content increased;differential scanning calorimetry(DSC)analysis showed that the hot press acid solution and hot press acid hydrolysis after enzymatic hydrolysis of starch samples of starch granule structure more closely,the unstable crystal structure,the differences in the internal structure of large.