Temporal And Spatial Variation Of Ozone In Beijing And Its Effects On Proteome Of Injured Leaves Of Ailanthus Altissima

The atmospheric ozone concentration increased sharply due to NOx and VOCs exhaust and became a serious eco-environmental problem.We monitored the ozone after2008 till 2015,and analyzed the temporal sequence.Mobile vehicle was used to monitor the ozone from urban to rural region in typical polluted day in summer in 2012.To discern the mechanism of proteome that influence leaves of plant,we simulate high concentration ozone in OTC with ozone generator and fan.The results is following:1)The ozone in Beijing after 2008 increased in trend,especially after 2013.2)In typical ozone polluted day in summer,the ozone concentration in the mountain region located in north-west of urban was higher than those in Teaching Botany Garden of Being,and those monitored on main road was lower than those in Teaching Botany Garden of Being.In spite of this,we monitored higher value than those in rural region in mountain.3)The special variance in mountain was much smaller than those on free way and main road of urban.4)Large scale uniform pressure field,pre-low cyclonic to total weather conditions causing air flow directed to north and resulted high ozone concentration in north mountain region of Beijing.5)The profiles of the protein expression in the leaf of Ailanthus altissima grown in O₃ stress or O₃ and drought stress were different from that of normal growing conditions.Under 40 and 80 ppb O₃ stress,there were 262 and 206 proteins respectively differentially expressed in Ailanthus altissima leaves compared with that of normal conditions,including 164 and 122 up-regulated proteins and 98 and 84 down-regulated proteins respectively.Under 40 and 80 ppb O₃ stress and drought stress,there were 95 and 125proteins respectively differentially expressed in Ailanthus altissima leaves compared with that of normal conditions,including 55 and 80 up-regulated proteins and 40 and 45down-regulated proteins respectively.These differentially expressed proteins were mainly related to carbohydrate metabolic,oxidative stress,photosynthesis,signal transduction and other biological functions.The differential expressions of the proteins related to photosynthesis may the results of the O₃ harm on the photosynthesis system.The differentially expressed proteins related to the oxidative stress may play important roles in responding to the oxidative stress caused by the O₃ stress.