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Design,Synthesis And Properties Of Fluorescent Probes Based On Rhodamine B

Posted on:2018-06-02Degree:MasterType:Thesis
Country:ChinaCandidate:R HuangFull Text:PDF
GTID:2381330512485861Subject:Physical chemistry
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Biothiols including glutathione,cysteine and homocysteine in organisms play important roles in individual live activities,their levels are the fundamental measure of health status.Therefore,qualitative and quantitative monitoring of their distribution in living systems could be significant to give more insight into their function.Organic small molecular fluorescent probes interact with the target molecules to generate fluorescence enhancement,fluorescence weakening or fluorescence peak displacement etc.Compared with other analysis methods,fluorescent probes have advantages with high sensitivity,low cost and wide choices,good reproducibility as well as convenient operation.Branches in different disciplines,chemsensors sustained rapidly development since they have been regarded as valuable tool in biochemistry,biological monitoring,biomarkers,disease detection and research of harmful substances process.The study and research of chemsensors is full of important practical significance.This thesis contains three chapters:Chapter 1:Background and introduction of fluorescent probe.Recent progress in the development of fluorescent probes for GSH.Chapter 2:A chemsensor,RhB-1,based on Rhodamine B has been designed and synthesized.This chemsensor is easy to synthesize and proved to be hydrosoluble,sensitive,selective and fast to sense GSH.Moreover RhB-1 have the ability to image in lysosomes of living cells.Chapter 3:A series of chemsensors,RhB-2 and RhB-3,have been reported.With RhB-1,three of the probes contain the same fluorophore(Rhodamine B)and reaction group but have the different link units.Experiencing to make sure the products.Based on these chemsensors,their structure-optical properties relationship of RhB-1,RhB-2 and RhB-3 have been evaluated.Three of the chemsensors all detect GSH by colorimetry and fluorescence enhancement.Since the emission wavelengths differ little,we supposed they detect in the same mechanism.Nonetheless,RhB-1 and RhB-3 react much faster than RhB-2 because of the influence of different linkers.In addition,the confocal pictures reveal there of the probes are able to image in in lysosomes of living cells.
Keywords/Search Tags:fluorescent probe, Rhodamine B, Glutathione, cell image
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