Targeted And Controlled Release Of HNTs Coupling SPOP Antibody Loaded Anticancer Drugs

Halloysite nanotubes(HNTs),a kind of natural nanomaterial with nanotubular structure and excellent biocompatibility,is a promising drug carrier for targeted tumor therapy.To research the targeting effect of HNTs loaded anti-cancer drug with cancer cells,we modified the surface of HNTs with bioactive molecules to make it with features of targeting carrier.Firstly,we modified the surface of HNTs with amination.Then we coupled the speckle-type POZ protein(SPOP)antibody to the surface of HNTs to establish the HNTs-SPOPab drug carrier and it was characterized by SEM?TEM?FTIR and XRD respectively.Secondly,we loaded the sorafenib(SOR)into HNTs-SPOPab and cocultured it with A498 cells for 24h.The inhibitory effect of HNTs-SPOPab loaded with drug was dected by FACS.Besides,we loaded gemcitabine(GEM)into HNTs to compare the inhibitory effect on A549 cells(without SPOP specific antigen)by Edu test.Results HNTs-SPOPab has features of stable structure and characters.The results of FACS suggested HNTs-SPOPab loaded with drug have more significant inhibitory effect to A498 cells.The percentage of suppression of HNTs-SPOPab loaded drug is higher(16.12 ± 0.45)%than free drug directly.The results of Edu test showed the HNTs-SPOPab loaded GEM have no significant difference with free drug to A549 cells.The suppression fluorescence ratio is HNTs-SPOPab-GEM;(94.12±3.91)%>GEM?(87.83 ± 1.42)%.To sum up,HNTs-SPOPab may be a potential promising targeting nanocarrier in the application of treatment in renal cancer.