Studies On The Preparation And Biological Activities Of Chondroitin Sulfate From Crocodile

Chondroitin sulfate,a kind of acid muco-polysaccharide,distribute in animal cartilage.We can get chondroitin sulfate through it by modern biotechnology,in the meantime we can get a good social value and economic benefits.The whole research study was aimed at investigating the preparation and bioactivities of chondroitin sulfate from the castoff of Crocodile processing.The appropriate extraction process was selected to produce Chondroitin sulfate.By studying single factor affected the yield in the process of alkali extraction and combining the response surface analysis to get the ideal extraction conditions which was 5%NaOH at 40°C for 209.82 min with the solid to solvent ratio in 1:14.We got a single material by using ion-exchange column chromatography to isolate and purify.According to chemical component analysis,the content of D-glucuronic acid,hexosamine and acidophobe was 26.05%±0.01,49.93%±0.01 and 26.03%±0.0l.Ultraviolet spectrum showed that the purified sample do not contain protein and nucleic acid.From the result of HPLC analysis further confirmed the purified sample is the chondroitin sulfate for which the retention time similar to the market of chondroitin sulfate.The result of IR analysis,the types of glycosidic bond in the purified sample was a-pyran glucose type.The structure of the purified sample was characterized 13C nuclear magnetic resonance.Cytology toxicology experiment showed that the crocodile chondroitin sulfate can promote the growth of L02 cells.To study the effect of immune regulation in vitro,the crocodile chondroitin sulfate can advance the proliferation of B-lymphocytes and T-lymphocytes.The results showed that the crocodile chondroitin sulfate with the antioxidant capacities by determining ABTS?,the reducing power and hydroxyl radical methods.The animal model,built by ICR mice,were applied to determine the anti-fatigued activities of Chondroitin sulfate and the serological test was taken to identify the mechanism.In this paper,different biological study methods were combined to research the antioxidant and anti-fatigued activities of Chondroitin sulfate from Crocodile in different aspects and level(molecular,cellular and individual),which laid the theoretical basement of processing Chondroitin sulfate,a by-product,into some kind of functional food to improve people's health level,reduced the pollution of the environment and had its sense on fully develop crocodile resource by making waste profitable.