Immunoassay Based On Nano-Materials Applied In Biomarker Detection

Screening and testing of biomarkers bring hope for the early diagnosis of cancers.Immunoassay has been applied to the detection of biomarkers due to easy operation and high sensitivity.Thousands of immunoassays based on different principles have been developed so far and each of them has pros and cons.For example,fluorescence immunoassay has high sensitivity,but fluorescence quench easily and background noise is strong at the same time.Therefore,we need to improve old methods and develop new immunoassays.Nanomaterials have excellent performance in chemistry,optics,magnetism and electricity,which lead to great use in immunoassays.Under this background,nanomaterials are introduced in our systems,with signal amplification and iron detection methods,we build three immunoassays for the detection of phospho-p53(S392)protein.1?The catalytic optical immunoassay of phospho-p53(S392)protein based on Fe3O4 nanoparticle.Fe3O4 nanoparticles have good stability,high tolerance to environment.What's more,the material itself is the amplification of the iron signal.Ferritin is called"water-soluble protein",which is also rich in iron.Wrapping the ferritin on Fe3O4 increases solubility and amplifies the signal.We build a new immunoassay of phospho-p53(S392)protein with Fe3O4-ferritin compounds synthesized.The signal of this immunoassay comes from the catalytic kinetic spectrophotometry detection of ferric iron released by the acid from the compounds.This method shows liner response with the range of 0.1 ng/mL-7.5 ng/mL and the detection limit is 0.08 ng/mL.2?The catalytic optical immunoassay of phospho-p53(S392)protein based on ferritin.Synthesis of cluster as a marker is also a signal amplification method used in immunoassay.Ferritin is rich in iron and can be connected to cluster.We build a new immunoassay of phospho-p53(S392)protein with ferritin cluster synthesized.The signal of this immunoassay comes from the catalytic kinetic spectrophotometry detection of ferric iron released by the acid from the ferritin cluster.This method shows liner response with the range of 0.5 ng/mL-10 ng/mL and the detection limit is 0.1 ng/mL.3?The optical immunoassay of phospho-p53(S392)protein based on detection of ferrous ion.The ortho phenanthroline photometric method is easier to operate and applicable to a wider range of pH relative to the catalytic kinetic spectrophotometry method.So in this section,we still use Fe3O4-ferritin compounds as the marker and build an immunoassay of phospho-p53(S392)protein.But signal detection method changes.We use the ortho phenanthroline photometric method to detect the ferrous ion,which is released by the acid from the compounds and reduced by the reducing agent.This method shows liner response with the range of 0.5 ng/mL-12 ng/mL and the detection limit is 0.2 ng/mL...