Fluorescent Detection Of Betalolol Hydrochloride Using Graphene Oxide And Functionalized Graphene

BackgroundGraphene with its unique and excellent physical and chemical properties,in the chemical sensors,biological detection,functional materials and other research areas have been widely developed and applied.The graphene oxide is an important derivative of graphene,and it has a larger?conjugated system.It can adsorb a fluorescent substance with aromatic structure by?-?conjugated stacking,which is adsorbed by fluorescence energy resonance transfer to produce fluorescence quenching phenomenon.A fluorescence based betalolol hydrochloride detection method has been developed using competitive adsorption interaction between fluorescent dyes and analyte on the surface of graphene oxide.However,for molecules without?-?stacking or weak?-?stacking,this method can not be used for detection.Therefore,a fluorescence based betalolol hydrochloride detection method has been developed basing on the competitive inclusion reaction between graphene bound?-cyclodextrin??-CD?with fluorescent dyes and analyte,comparative study of functionalized and non-functional graphene-based detection of target molecules.Objective?1?To design a novel graphene oxide-based fluorescence sensor for betaxolol hydrochloride detection in Plasma;?2?To establish a novel fluorescence sensor for betaxolol hydrochloride detection and recognition directly,using?-cyclodextrin and HP-?-cyclodextrin modified functionalized-reduced graphene oxide?rGO?-based fluorescence sensor.Method?1?Fluorescent dye can been bound on GO to form FD/GO complex for the?-?stacking,then the following fluorescence resonance energy transfer?FRET?results in high-efficiency fluorescence quenching?‘turn-off'?.Competitive adsorption exists between fluorescent dyes?rhodamine 6G,R6G?and analyte?betaxolol hydrochloride?.When the binding intensity of fluorescent dyes is weaker than of BH,fluorescent dyes can been replaced by BH and fluorescence enhance?‘turn-on'?;?2?Functionalized-graphene??-CD@rGO?and?HP-?-CD@rGO?was characterized by fourier transform infrared spectrum?FT-IR?,UV spectrophotometry?UV-vis?and thermogravimetric analysis/differential scanning calorimetry?TGA/DSC?.Fluorescence of?-CD@rGO and HP-?-CD@rGO incorporated R6G is quenched by graphene but is‘turned on'by betaxolol hydrochoride as it replaces R6G from the?-CD or HP-?-CD host,determination of fluorescence intensity ratio to achieve the detection of betaxolol hydrochloride.Result?1?A good linear relationship was showed between the fluorescence intensity ratio?I-I₀?/I₀ and concentrations of BH in fetal bovine serum ranging from 1.0 to 10ìmol/L;?2?FT-IR,UV-vis and TGA/DSC suggested that the?-CD or HP-?-CD were modified to graphene successfully.And functionalized-graphene-based fluorescence sensor was applied to the detection and recognition of betaxolol hydrochloride,especially HP-?-CD@rGO recognition of betaxolol hydrochloride was superior to?-CD@rGO recognition of betaxolol hydrochloride;the fluorescence intensity ratio?I-I₀?/I₀ and concentrations of BH ranging from 1.0 to 10.0?mol/L have a linear relationship for HP-?-CD@rGO recognition of betaxolol hydrochloride,the limit of quantitation of the two kinds of functionalized-graphene-based fluorescence sensor for betaxolol hydrochloride were 5.0 nmol/L.Conclusion?1?R6G/GO fluorescence sensor has been applied to the determination of the content of betaxolol hydrochloride in fetal bovine serum quickly and sensitively.R6G/GO fluorescence sensor have good specificity and selectivity for the detection of betaxolol hydrochloride in the presence of different interfering substances;?2?Functionalized-graphene-based fluorescence sensor for the detection of betaxolol hydrochloride than non-functional graphene fluorescence sensor has a strong anti-interference ability and high sensitivity;two kinds of functionalized-graphene-based fluorescence sensor all realized the detection of betaxolol hydrochloride,fourteen kinds of other substances have not almostly interfered in the detection of betaxolol hydrochloride.