Investigation On Ionic Liquid Functionalized Interfaces Construction And Their Applications In The Electrochemical Sensing Of Tumor Markers

Ionic liquids possess good physical and chemical properties,and have been widely used in the field of energy,catalysis,and sensing etc.When ionic liquids were polymerized,conductive polymers will be obtained with the inherit properties of ionic liquid.In addition,some properties of polymers such as chemical stability,durability and physical rigidity also have been endowed to the polymerized ionic liquids.Thus,in the recent years,the polymerized ionic liquids have emerged as a promising research field.The main contents of this dissertation are presented as following: 1.3-Amine-N-[3-(N-pyrrole)propyl]imidazole bromide ionic liquid(APPIBr)has been successfully synthesized.The as-synthesized ionic liquid was confirmed by 1H NMR,HPLC-MS,FTIR,and UV-vis.APPIBr possesses a pyrrole moiety which can be polymerized by using electrochemical technique.Moreover,amine groups will supply some active sites for immobilizing biomolecules to fabricate biosensors.2.Using chloroauric acid as precursor,gold nanoroots(Au NRs)were fabricated by using dodecyl imidazole as functional monomer.The obtained Au NRs were characterized with TEM,SEM and XRD.Then,Au NRs were drop coated onto a glassy carbon electrode surface to fabricate a nanointerface,eg.Au NRs/GCE.Subsequently,APPIBr ionic liquid was electrochemically polymerized onto Au NRs/GCE surface.After electropolymerization,squamous cell carcinoma antibody was immobilized on the poly(APPIBr)/Au NRs/GCE surface to fabricated an electrochemical immunosensor.It was found that poly(APPIBr)/Au NRs nanointerface can improve the sensing performance of the immunosensor.Under the optimized experimental conditions,the peak current variation is linearly related to squamous cell carcinoma antigen concentration in the range of 0.001~0.1 ng m L-1 and 0.1~5.0 ng m L-1.The detection limit is calculated to be 0.3 pg m L-1? 3.Gold nanoparticles(Au NPs)were electrochemically deposited onto a glassy carbon electrode surface,and was used as a substrate to assemble 1-[3-(N-cytimine)propyl]-3-vinylimidazolium tetrafluoroborate ionic liquid [(Cyt)VIMBF4]to fabricate nanocomposite interface [(Cyt)VIMBF4-Au NPs/GCE].By using(Cyt)VIMBF4 ionic liquid as functional monomer,alpha-fetoprotein(AFP)as a template,ethylene glycol dimethyl acrylate as a crosslinking agent,ammoniumsulfate and tetramethyl ethylenediamine as initiator,a polymer film can be formed on the(Cyt)VIMBF4-Au NPs/GCE surface under room temperature.After removing template,an AFP imprinted electrochemical sensor can be developed.The as-prepared molecularly imprinted sensor exhibits excellent selectivity towards AFP,and can be used for sensitive determination of AFP.Under the optimal conditions,the imprinted sensor shows a good linear response to AFP in the concentration range of 0.03 ng m L-1 ~ 5.0 ng m L-1.The detection limit is estimated to be 2 pg m L-1?...