| The integration of diagnosis and treatment of diseases has become a tendency in the field of biomedicine study.The emergence of multi-functional nanoparticles has made the idea of integration of diagnosis and treatment a reality.To accomplish effective cancer imaging and integrated therapy,the multifunctional nanotheranostic Fe3O4-MTX@HBc core-shell nanoparticles(NPs)were designed.Firstly,MTX was chemically attached to the surface of Fe3O4 nanoparticles and chelated with Ni2+.In the process of disassembly and self-assembly of HBc VLPs,an affinity for methotrexate chelate(MTX)-Ni2+ by the histidine tag in the structure is achieved,and magnetic Fe3O4-MTX nanoparticles can be effectively encapsulated into genetically engineered virus-like particles in a straightforward way.HBci44-His VLPs shell could protect Fe3O4-MTX NPs from the recognition by the reticuloendothelial system as well as could increase their cellular uptake efficiency.Through the in vitro characterization of Fe3O4-MTX@HBc nanoparticle by MRI and photothermal performance,the results showed that the material presented very good MRI imaging capability and photothermal conversion efficiency.Based on the good performance of Fe3O4-MTX@HBc nanoparticles in vitro,we subsequently performed MRI of Fe3O4-MTX@HBc nanoparticles in mouse breast cancer 4T1 cell line and mouse breast cancer heterotopic xenograft model,respectively.The ability and combined anti-tumor effects were evaluated and Fe3O4-MTX@HBc nanoparticles exhibited ideal chemotherapy in both in vivo and in vitro experiments under near-infrared(NIR)laser irradiation using our well-designed combination photothermal/chemotherapy.Moreover,Magnetic resonance imaging(MRI)results showed that the Fe3O4-MTX@HBc core-shell NPs were reliable T2-type MRI contrast agents for tumor imaging.Hence,the Fe3O4-MTX@HBc core-shell NPs may act as a promising theranostic platform for multimodal cancer treatment. |
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