| Malignant tumor,which has become one of the thorny public health problems in the world,is a great threat to human health.The emergence of nanomedicine is the perfect combination of nanotechnology research and biomedical research,and provides a new approach and strategy for tumor therapy.Recent studies have shown that fullerol nano drugs,as carbon based model nanomaterials,have demonstrated excellent performance in the respect of selectivity of cancer cells,reducing damage to normal cells and treating the tumor.Based on the mentioned above,the master's thesis carried out the research on the preparation of fullerol nano drugs and its cytotoxicity,and ultimately achieved results as followed:1.Fullerol-silica nanocomposite was synthesized by the combination of Fullerol and silicon dioxide.According to the property of the negative electricity on the surface of Fullerol,the experiment selected the silicon dioxide with negatively charged surface as the core,and the positive PDDA(Polydiallyl two methyl ammonium chloride)as the surface active agent,thus preparing the Fullerol-silica nanocomposites by utilizing the electrostatic interaction between different charges.The optimum conditions for preparing monodisperse Fullerol-silica nanocomposites were determined by means of DLS and Zeta potential.2.The cytotoxicity of the synthesized Fullerol-silica nanocomposite drugs was studied.The result shows that when the concentration of Fullerol nanoscale was lower than 50 ppm,the cell value-added rate was more than eighty percent,that is,the drug concentration produced relatively low toxicity to the cells.3.The reaction between Fullerol-silica nanocomposites and the main digestive enzymes in human body was preliminarily explored by means of nano stress sensing and surface enhanced Raman scattering.As the blank control group also produced the peak position of the movement,however,the preliminary result is not clear whether the Fullerol nanomedicine could react with trypsin and pepsin in human body. |
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