The Application Of Photosensitive Catalytic Oxidation Method For Removing Malachite Green In The Water And BRCA1 Sensor

In recent years,photocatalytic technology has become one of the world's most hot research topics,currently and usually it real applications was evaluated in environment and energy field.Expecially in the study of water treatment,air purification and other aspects.Photosensitive catalytic oxidation method as one of photocatalytic oxidation technology,it has seveal unique advantages such as simple device,avalibility of various light soucrce,easy operation,application widely.Thus,it has been extensively studied in water treatment and biomedicine.In our work,Photosensitive catalytic oxidation method combined with nanomaterials graphene oxide?GO?applied to treat fungicide in water,and applied to BRCA1cheniluminescence sensor research.The main contents are as follows:1.GO photosensitive catalytic oxidation method was used to remove malachite green in water.Through spectral characterization of GO,we studied the photoluminescence performance and functional group of GO.Due to the large amounts of oxygen functional groups on the surface of GO,it was dispersesed easily in water.Under photosensitive catalytic oxidation method,GO removed malachite green in water effectively,the mechanism of removing fungicide were investigated.The effects of GO concentration,removal time,system pH,malachite green concentration were tested.From the results,we found that pH has little effect in the system;the increase of GO concentration and removal time can enhance removal rates.When GO concentration was 0.1 mg/mL and illumination time was 120 min,the removal efffiency was as high as 94.4%.In real water sample,removal efficiency was can reach 81.3%.Therefore,GO-based photosensitive catalytic oxidation method could remove malachite green in water effectively.The method is feasible and it has advantages of avalibility of various light soucrce,simple operation,assembling easily.2.The feasibility of exploring Fe₃O₄/GO materials for removing malachite green in water was studied.By using coprecipitation method and expoliting nano-materials GO as matrix,Fe₃O₄/GO composites nanoparticles were prepared.Through spectral characterization of Fe₃O₄/GO,it was found that Fe₃O₄/GO has abundant functional groups.Using 216?g/mL Fe₃O₄/GO to remove 10?g/mL malachite green,the adsorption efficiency was 98.7%;By the asistance of photosensitive catalytic oxidation method,malachite green removal efficiency reached 99.2%under the same conditions.The rest concentration of malachite green after photocatalytic treatment was 60%lower than that after absorption only.Preliminary tests showed that Fe₃O₄/GO under Photosensitive catalytic oxidation method has a higher removal efficiency in removing malachite green.3.By combining photosensitive catalytic oxidation method of the dye molecules with BRCA1 sensing,a new light-emitting sensor technology was developed.The cyanine dye TOTO-1 could be embedded into double-stranded DNA?dsDNA?,and after LED illumination,the complex can catalyze the oxidation of luminol with the generation of CL signal.The catalytic activity can be greatly enhanced after TOTO-1embedded dsDNA.We investigated the effect of different dyes,pH,incubation time,illumination time and TOTO-1 concentration.Using this method,breast cancer gene?BRCA1?detection can be achived in the range of of 0.01 nmol/L-5 nmol/L with a correlation coefficient of 0.9958.The dection limit was as low as 3 pmol/L.In real serum sample,the anti-oxidation substances could inhibit the luminol oxidation by dsDNA-TOTO.Carboxyl UF Magnetic Particles?CUFMP?,which is rich in carboxyl?-COOH?react with DNA modified by amino were used for elimation of the interference by seperating the target DNA from the serum.In this way,BRCA1 gene detection was linear in the range of 0.5-100 nom/L and the correlation coefficient is0.9951,the dection limit was as low as 0.17 nmol/L,it has better recovery rate,which is 93.8%-99.0%.