Preparation And Characterization Of Functionalized Mesoporous Nanoparticles And Their Applications

With the increasing development of nanoscience and nanotechnology,more and more attention has been drawn on the biomaterials.Among them,mesoporous materials have been largely used in biomedicine field because of the high surface areas and outstanding physicochemical properties.Mesoporous silica nanoparticles were firstly used as drug carriers and functionalization was necessary to improve the performance of the nanocarriers.In the past decades,mesoporous titania have emerged to be another alternate to perform as drug carriers due to its special photoelectric properties.However,the lack of cell-targeted of anticancer drugs and undesired release rates may limit the further clinical application.As a result,the introduction of cell-targeted mechanism is important.In addition,the Pickering emulsion using nanoparticles as surfactants has drawn much attention recently.Firstly,two kinds of mesoporous silica nanoparticles with different pore size distribution have been fabricated by using two different surfactants.Then,amino groups were grafted on the surface of the channels.VE was loaded through recrystallization inside the channels by electrostatic attraction.The novel drug nanosystems can protect the VE from oxidation,as well as the pH-responsive release properties.The obtained nanosystem were characterized by FTIR,BET,TEM,TGA,DTA and UV-vis reflection.The formation mechanism was also proposed and verified.The cytotoxicity was also tested by CCK8,Hoechst stain and hemolytic tests.The results showed that the nanosystem possessed excellent biocompatibility,which can be widely used in food industry,cosmetics and biomedical field.Secondly,two kinds of mesoporous titania nanoparticles with different structures including MTN and HTMN were successfully prepared using organic amine as templates.Then,PEI was grafted on the surface of the mesoporous titania nanoparticles through electrostatic attraction.FA was then linked on the surface through the reaction between–COOH and–NH₂.Cur was successfully encapsulated inside the channles to obtain the novel anticancer nanosystems with cell-targeted properties.In particular,when mesoporous titania was exposed to the UV light,ROSs which possess strong oxidizability were generated.After PEI which blocked the mesoporous channles were decomposed,Cur molecules were released at a higher rate.In addition,through the characterization such as XRD,FTIR,BET,TGA and DTA,the best preparation parameters of HMTN was found out and the formation mechanism was also discussed.After the evaluation of cell experiments,the biocompatibility of the novel drug systems was proved.Thirdly,Pickering emulsion loaded with IBU was successfully prepared using mesoporous titania nanoparticles as surfactants,IPM as oil phase,carbomer as thickener,Tween and Span as cosurfactants.In addition,various factors including sizes and amounts of particles,oil-water ratio were assessed.The obtained product,which was O/W emulsion,have promising future in the skin care fields.