| The development of selective,safe and resistant microbial pesticides has become the focus of the industry and pesticide industry.The ht-q6 was separated from the walnut skin and was inducted into a highly effective mutant strain.Based on the strain for material,this paper developed the wettable powder and emulsion suspension agent,the agent and determination of two kinds of bacteria of tomato field efficacy,lay a foundation for its application in production.1.The Bacillus amyloliquefaciens GHt-q6 fermentation liquid and diatomaceous earth mixed and dried by grinding to get the powder,through the screening of additives to determine the Bacillus amyloliquefaciens GHt-q6 wettable powder formula:carrier for the diatom(85%),the wetting agent is SDBS(6%),the dispersant is sodium lignosulfonate(6%),the stabilizer is KHP04(1.0%),the UV protectant is Vc ascorbic acid(1.0%).The quality detection showed that the spore content of Bacillus amyloiquefaciens GHt-q6 wettable powder is 2.28×109cfu·g-1,the rate of bacteria was 0.5%,the pH value was 5.8,the fineness was 93.5%,the dry reduction was 2.5%,wetting time was 45s,the suspension rate was 85.6%,in line with the national standard value.The effective storage time was about 90 days,and the effect was best within 60 days.But also to the tomato plants without pathogenicity,the acute oral toxicity of rats are low toxicity,percutaneous toxicity is a low toxicity,the rabbit skin irritation,the indicators qualified,can be used for field trials2.Through the screening of additives to determine the Bacillus amyloliquefaciens GHt-q6 emulsion suspension formula:the solvent oil was peanut oil(10%),the emulsifier was Tween 80 and OP-10.(0.4%),the wetting agent is SDBS(6%),the stabilizer is KHPO4(1.0%),the UV protectant is Vc ascorbic acid(1.0%).the antibiotic is nystatin(25?g · mL-1),the antifreeze is ethylene glycol(6%).The quality detection showed that the spore content of Bacillus amyloiquefaciens GHt-q6 emulsion suspension is 2.54×109cfu·g-1,the rate of bacteria was 0.85%,the pH value was 7.2,the fineness was 96.5%,the suspension rate was 90.5%,the dumping,low temperature stability and thermal storage stability are in line with national standard values.The effective storage time of the emulsion powder was about 80 days,and the effect was best within 54 days.But also to the tomato plants without pathogenicity,the acute oral toxicity of rats are low toxicity,percutaneous toxicity is a low toxicity,the rabbit skin irritation,the indicators qualified,can be used for field trials3.Of Bacillus amyloliquefaciens GHt-q6 wettable powder and emulsion suspension were measured in laboratory and field experiments,the virulence of several pathogenic fungi were determined,results showed that the biocontrol agent WP comparison on Botrytis cinerea inhibitory effect with other pathogenic fungi;and suspensions have no obvious difference four kinds of fungus disease suppression effect,for the same kind of pathogenic fungi wettable powder was better antibacterial effect.To test the control effect of different concentration,dosage form of Botrytis cinerea and Fulvia fulva.The results show that the control effect of wettable powder with 50x dilution multiples on Botrytis cinerea 76.6%,Fulvia fulva the control effect reached 74.1%,the control effect is more significant than the emulsion suspension,difference and control effect of the control agent is not significant,but the control agent for chemical agents,it is recommended to use the dose in the production practice of wettable powder with 50x dilution multiples to the prevention and control of Botrytis cinerea and Fulvia fulva. |
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