Effect Of Polyphenols From Physalis Fruit On Glucose Metabolism In Type ? Diabetic Mice

[Objective]The hypoglycemic effect of Physalis fruit polyphenols on type II diabetes in mice was investigated,and to further explore the hypoglycemic mechanism of Physalis fruit polyphenols.[Methods]SPF male ICR mice fed for 7 d,were randomly divided into normal control group(basic Plastic)and model group(high fat diet),4 weeks after,fasting for 12 h,the bacterial body mass by intraperitoneal injection of 60 mg/kg streptomycin,three days later,fasting for 12 h,the bacterial body mass by intraperitoneal injection of 60 mg/kg streptomycin,fasting for 12h,tail vein blood of mice was measured in fasting blood glucose,fasting blood glucose was determined as 11.10mmol/L mice model.The 50 experimental animals were randomly divided into 5 groups after the success of modeling:Physalis fruit polyphenols in high dose group(1.50 g/kg/d),Physalis fruit polyphenols in middle dose group(0.75 g/kg/d),Physalis fruit polyphenols in low dose group 0.30 g/kg/d),positive control group(0.75 g/kg/d metformin hydrochloride),model group(saline),and measured body weight and fasting blood glucose every week.After 28 days,mice were measured glucose tolerance,organ index,serum Triglyceride(TG),Total cholesterol(TC),High Density Lipoprotein Cholesterol(HDL-C),Low Density Lipoprotein Cholesterol(LDL-C),Alanine Aminotransferase(ALT),Aspartate Aminotransferase(AST),The liver,kidney,and pancreas were prepared for routine HE staining and the kit was used to determine hepatic glycogen,the activity of hexokinase(HK),pyruvatekinase(PK),glucose-6-phosphatase(G6Pase)and phosphoenolpyruvate carboxykinase in liver tissues.the relative expression of glucose transporter2(GLUT2),G6Pase,PEPCK,PK,and glucokinase(GK)mRNA in liver tissue were measured by real-time RT-PCR.[results]1.Physalis fruit polyphenols can reduce the weight loss of type ? diabetes mice,improve glucose tolerance and reduce the liver,kidney,pancreas index,spleen index increased;decrease the levels of TG,TC and LDL-C,improve the level of HDL-C;reduce the level of ALT and AST,and the effect of Physalis fruit polyphenols was positively related to the dose;the results of all the indexes showed that Physalis fruit polyphenols in high dose group had the best effect.2.The histopathological section showed that the pathological changes of the liver,kidney and pancreas of type ? diabetic mice were alleviated in different doses by physalis fruit polyphenols at different doses,and the degree of remission was slightly higher in the high dose group than in the middle and low dose groups.Compared with the model group,the physalis fruit polyphenols at different doses had different degrees of improvement on the fatty vacuoles,the arrangement,the contour and the structure of the liver of the type ? diabetic mice,and the changes in the glomerular volume and the integrity of the glomerular basement membrane in the renal tissue;the arrangement of pancreatic islet cells.Contour clarity was improved,and the positive control group and the high dose group of physalis fruit improved significantly.3.Physalis fruit polyphenols can increase hepatic glycogen content,increased the activity of HK and PK,decreased the activity of G6Pase and PEPCK in the liver of test mice4.Physalis fruit polyphenols can increase the expression of GK,PK and PEPCK mRNA,reduce the expression of G6Pase and PEPCK mRNA in the liver of test mice.[Conclusion]Physalis fruit polyphenols can control the activity of related enzymes in liver tissue by up-regulating the expression of GK,PK and PEPCK,and down-regulating the expression of G6Pase and PEPCK in liver tissue,thus improving the glucose metabolism in diabetic mice and further reducing the blood glucose levels,and ultimately reaching the purpose of lowering blood glocose..