Bioactivity Efficacy Verification For Lycium Barbarum L.polysaccharide Conjugate And Its Combination With Grape Seed Polyphenols

Lycium barbarum L.polysaccharide conjugate refined from Lycium barbarum L.polysaccharide.In this studies,we studied the immunomodulatory of LBP and investigated the effect of the mixture of LBP and grape seed polyphenols(GSP)on immunomodulatory.The main research is concluded as follows:(1)We measured the composition of LBP.The result indiciated that it mainly contains polysaccharide,uronic acid and protein at 55.45%,5.72%,and 28.58%,respectively.Fourier transform infrared proved that LBP has a typical polysaccharide absorption pattern and the?-(1?6)-or ?-(1?3)-linked galactan and arabinose in side chains.Monosaccharide composition analysis,which measured by HPLC and GC,showed that LBP consisted of Rha,Ara,Man,Glu,Gal and Gal A.The ratio of Rha,Ara,Man,Glu,and Gal was 1.09:1.40:0.86:1:0.08.(2)Antioxidant activity is closely related to immunomodulatory.LBP have stronger DPPH and ABTS radical scavenging activity rather than OH.It means that LBP has better radical scavenging activity of reactive nitrogen species than reactive oxygen species.(3)The immunomodulatory of LBP in vitro was tested by measuring the phagocytic activity of macrophages and the proliferation rate of splenocytes.It was shown that LBP of 450 to 600?g/mL can increase phagocytosis of macrophages in a dose-dependent manner.Besides,LBP can increase the proliferation of spleen lymphocytes by regulating the production of TNF-a in spleen cells in a dose-dependent manner.1200 ?g/mL is the optimal concentration of LBP to increase the proliferation rate of spleen cells.(4)The mouse immunosuppression model was established by the injection of cyclophosphamide to evaluate the immunomodulatory of LBP in vivo.It was shown that LBP has a positive regulatory effect on the recovery of white blood cell counts,organ index,mononuclear-macrophage phagocytic activity,natural killer cell-mediated cytotoxicity and splenic T and B cells proliferation in immunosuppressed mice.Due to LBP contains a lot of arabinose,it has a stronger ability to enhance the proliferative activity of B cells than T cells.(5)In this study,LBP was mixed with GSP to investigate the combination effect on immunomodulatory activity compared to the single component.The mixture has a synergistic effect on DPPH free radical scavenging activity rather than ABTS free radical scavenging activity.In terms of immunomodulatory in vitro,the proliferation of splenocytes was strongly stimulated after treatment with the mixture of LBP and GSP.The mixture strongly increased the intracellular free calcium levels in mouse splenocytes.In terms of immunomodulatory in vivo,the mixture significantly enhanced the immunity of immunosuppressed mice,as evident from the significant increase in peripheral white blood cell count and B cell proliferation.The changes in the spatial structure of the sugar chain were related to the synergistic effect of the two functional agents on immune recovery.