Preparation Of Medical Hydrogel Loaded With Bioantibacterial Microsphere

At present,the most widely used antibacterial agents on the market include nano silver,graphene,etc.,which have good antibacterial effect,but have potential harm to human cells.Individual patients may be allergic to ingredients,and nano silver,graphene and the like are expensive.The subject of this present study uses a natural bio-antibacterial and anti-allergic drug,naringenin,which is extracted from waste resources such as grapefruit peel.It has a wide range of sources and can promote the rational use of resources.This present study firstly prepared naringenin/sodium alginate/chitosan microspheres by high-pressure electrostatic spray method,then prepared PVA/ODex hydrogel by microwave assisted method,and finally naringenin/sodium alginate/chitosan micro the ball was loaded into a PVA/ODex hydrogel to prepare a naringenin/sodium alginate/chitosan microsphere PVA/ODex composite hydrogel.(1)Explore the process parameters of preparing naringenin microspheres by high-voltage electrostatic spray method,that is,the voltage of high-voltage power supply is 16 kV,the speed of propeller is 5 mL/h,the concentration of calcium chloride is 2%,and the concentration of chitosan 0.03%,the mass ratio of sodium alginate to naringenin is 1:10,the pH of the solidified liquid is 5.5,and the surface morphology,particle size,swelling property of naringenin/sodium alginate/chitosan microspheres,Degradation performance and drug release performance were explored.It was found that the microspheres were spherical and uniform in size;the swelling degree of microspheres was up to 25 times;the microspheres were degradable,and the degradation rate was 12.8% when degraded;The release rate was faster in the first 48 h,and the release amount gradually became stable and stable over time,and the percentage of naringenin/sodium alginate/chitosan microspheres was more than 70%.(2)The naringenin/sodium alginate/chitosan microsphere PVA/ODex composite hydrogel was successfully prepared by microwave assisted method.The surface morphology of the naringenin microsphere hydrogel was firstly observed by scanning electron microscopy.Characterization was performed and it was observed that the naringenin microspheres were distributed in the three-dimensional network structure of the PVA/ODex hydrogel.Secondly,the naringenin microsphere hydrogel was tested by infrared.The characteristic absorption peaks were found at 1627.89,1416.28,1087.27,815.77,560.28 and 478.17 cm-1,which verified the successful loading of the hydrogel.Finally,the mechanical properties,swelling properties and degradation properties of the naringenin microsphere hydrogel were studied.The amount of naringenin/sodium alginate/chitosan microspheres was found to be naringenin/sodium alginate/shell poly The mechanical properties,swelling properties and degradation properties of the sugar microsphere PVA/ODex composite hydrogel have an effect.The mechanical properties and degradation properties of the microspheres decrease with the addition of microspheres,but with the addition of microspheres.Increasing its swelling performance gradually increased.With the increase of the amount of naringenin/sodium alginate/chitosan microspheres,the degradation of naringenin/sodium alginate/chitosan microsphere PVA/ODex composite hydrogel fast.(3)Characterization of the structure of PVA/ODex composite hydrogel loaded with naringenin/sodium alginate/chitosan microspheres.The results showed that the addition of naringenin/sodium alginate/chitosan microspheres made PVA /ODex hydrogel showed excellent inhibition against Gram-positive bacteria and Gram-negative bacteria,and its inhibition rate reached 96% and 92%,respectively,and showed good hyaluronidase inhibition performance in vitro.The inhibition rate was 52.42%.