| Aiming at rapid and on-site detection of trace sulfonamides(SAs)and quinolones(QNs)in water environment,the determination technique was established based on Surface-enhanced Raman spectroscopy(SERS).The proposed protocol is simple and easy to operate,which satisifies the detection levels of surface and drinking water samples,and therefore is a promising on-site detection tool of trace antibiotics in water environment.Highlights of current research are summarized as follows.(1)The qualitative and quantitative analysis of SAs(sulfadiazine,sulfamerazine and sulfamethazine)was developed with gold nanoparticles(Au NPs,55.5 nm in diameter)as the SERS substrate.The limit of detection(LOD)was 0.08,0.06 or 0.12μg/L,with the linear range was 1.0-100.0,50.0-1000.0 and 50.0-1000.0μg/L for these three SAs,respectively.Facilitating by filteration as pretreatment,rapid detection of trace SAs in lake,fish pond,aquaculture wastewater and other water samples were carried out directly with the varied recovery of 62.7%-110.0%.(2)This chapter developed a rapid qualitative and quantitative microanalysis method for QNs(enrofloxacin and ciprofloxacin)based on SERS technology with silver nanoparticles(Ag NPs,60.0 nm in diameter)as SERS substrate.The LODs were both 0.01 μg/L,and the linear ranges were both 0.05-10.0 μg/L.This study confirmed that the rapid detection of QNs in lake water,fish pond water,aquaculture wastewater and other water samples could be realized without pretreatment,with the recovery of 63.9%-115.0%.(3)To pushing the detection level down to ng/L level,magnetic solid phase extraction(MSPE)was introduction as the enrichment pretreatment step.Under the optimized conditions,the LODs were 0.31 and 0.16 ng/L,with the linear ranges of 1.0-1000.0 and 5.0-1000.0 ng/L for these two QNs investigated,respectively.The feasibility of trace QNs detection in tap water was explored at ng/L level with the recovery of 62.7%-110.0%.It should be noted that,the selectivity of MSPE is not good enough facing water samples(such as lake)containing complicated organics.Nevertheless,this method provides a rapid,sensitive and efficient method for the detection of trace QNs in drinking water with simple matrix,which displayed the high selectivity towards QNs over SAs. |
