Synthesis,Characterization And Biocompatibility Of Aluminum Oxyhydroxide Nanoparticles

With the development of nanotechnology,more and more application of nanometer materials in our daily life,especially life sciences and pharmaceutical applications have been widely paid more and more attentions and made great progress in the field of nanotechnology.Inorganic nanomaterials,especially nanomaterials that have specific morphologies or sizes,have brought new insight for drug release and precision treatments systems.Therefore,the research on the morphology,size and the directional design of nanomaterials are becoming more and more important.AlOOH has the advantages of higher specific surface area,larger contamintcapture capacity,large porosity,and good dispersibility,such as rich adjustable dimensions,morphology,etc.Therefore,the nanostructure material have been considered as excellent properties and broad prospects in the fields,such as,adsorptiont,catalyst and carrier,composite materials,biological separation,biological detection,proteolytic digestion,biological sensors and biological medicine and so on.In this thesis,we have designed AlOOH nanoparticles of different morphology and size,the interaction between AlOOH and cells,proteins have been studied,different morphology,size and other properties of nanomaterials to cytotoxicity,cell uptakes,protein adsorption,have been revealed,which provides a new angle of view on AlOOH materials in biological applications.First of all,we have synthesized rod and flake AlOOH nanoparticles by hydrothermal method,carried on the characterization to the nanocomposites,exploresd the effect of fluid temperature,reaction time,aluminum water source,solvent volume ratio and pH value on morphology,size and dispersion,and the mechanism of AlOOH nanomaterials has carried on the brieft discussion.The results shows that,on the condition of 150 ?,18 h,volume ratio of 1:1 volume,the morphology and size of AlOOH nanoparticles are uniform,and when pH<7,will form a rod shape,when pH>7,will form a flake AlOOH.Then,we have discussed the effect of different AlOOH nanoparticles shape and sizes on Raw264.7 cells and Hela cells including MTT,ROS level,cell morphology and cell uptake etc.The results showed that the nanoparticles in the morphology were more easily reunite in the medium,which could be more difficult to be uptaked by cells than the rods.The effect of AlOOH nanoparticles on cell viability was dose and morphology dependence.The intracellular ROS level increased with higher concentration,and decreased the cell vitality,but the biocompatibility is still better.In the end,we compared the rod and flake AlOOH particles for protein adsorption,adsorption factors,found that adsorption time,initial concentration of OVA,pH value,adsorption OVA for AlOOH has obvious effect.The study shows that the optimal ratio between OVA and AlOOH,namely,the mass concentration ratio of OVA and AlOOH is 5:1,at which time the adsorption capacity of AlOOH reaches the maximum,and the adsorption equilibrium is only one hour.