Study On The Efficacy And Mechanism Of Microalgae Derived Active Peptides In The Kidney Protect

Diabetes and its derived diabetic nephropathy is a common disease of human beings.Once it develops to the end stage of kidney disease,it will greatly threaten the life and health of patients.Therefore,it is important to prevent diabetic nephropathy in time.At present,most of the drugs that treat and prevent diabetic nephropathy have side effects.Therefore,non-toxic side effects and effective protection of new kidneys are urgently needed.This project is mainly concerned with the renal protection effect of bioactive peptide LHY7 in diabetic mice,and preliminarily explores its mechanism of renal protection.First of all,bioinformatics method was applied to predict and analyze the hydrolysate peptides from S.pomaciaca,and some functional oligopeptides were screened out.Next,we carried out in vitro efficacy tests of target bioactivity.A preliminary study on glomerular mesangial cells(SV40 MES 13)was made by enzymolysis peptides.The results showed that the inhibition rate was obvious.The results showed that the inhibitory rates of the three bioactive peptides labeled as 2-12,LHY7 and LHI7 were the highest.Then,the three bioactive peptides were prepared into five concentration gradient solutions of 1,0.1,0.01,0.001 and 0.0001 mg/mL,respectively,and cultured respectively.The results showed that the inhibitory effect of the bioactive peptide on cell proliferation was concentration dependent.After 48 h cell culture,when the concentration of active peptide was 0.1 mg/mL,the inhibitory rate of 2-12,LHY7,LHI7 active peptide was relatively high,and the inhibition rate of LHY7 was the highest,which was 46%.It can be seen that LHY7 active peptide has a certain inhibitory effect on cell proliferation,and the most suitable concentration is 0.1mg/mL.Second,the effects of bioactive peptide LHY7,when the concentration of active peptide was 0.1 mg/mL on body weight,blood sugar,kidney physiological index and renal tissue were studied by intraperitoneal injection in the model of type I diabetic mice.The results showed that the renal organ coefficient of mice given by intraperitoneal injection was significantly lower than that of the model group,which showed that the active peptide could inhibit the renal hypertrophy to some extent,and the body weight of the active peptide group increased in LHY7.By measuring the blood sugar of mice,it was significantly decreased with the before and the model group.It showed that the active peptide had the effect of reducing blood sugar,the measurement of urine microalbuminuria,serum urea nitrogen and creatinine in mice showed that the active peptide could delay the damage of renal function to a certain extent.The postoperative pathological changes were significantly lower than those in the model group.Third,at the gene level,the expression of ACE and ACE2 of renal injury related regulators in type I diabetic mice was analyzed by semi quantitative PCR and Q-PCR.Semi quantitative PCR showed that the expression of ACE decreased,and the expression of ACE2 increased.In order to study more accurately the effect of active peptide on various regulatory factors,Q-PCR was carried out.The results showed that the expression of ACE in diabetic mice treated with LHY7 was significantly lower than that in the model group,and the expression of ACE2 increased significantly.Therefore,bioactive peptides can reduce renal damage by regulating gene expression.