Design,Synthesis And Biological Application Of Dual-Site Fluorescent Probe

Active species play vital roles in various physiological and pathological processes,and are involved in signal transduction,differentiation,migration and cellular immunity,closely associated with the occurrence and development of related diseases.So,it is of great importance to developed in situ detection methods for active species with high sensitivity,high selectivity and rapid response time.Information about distribution,change in concentration of active species is conducive to study the molecular mechanism of relevant physiological and pathological processes.In this study,by proteases overexpressed in cancer cells(such as nitroreductase,NRT and human quinone reductase1,hNQO1)as the research object,based on fluorescence dye in situ generation strategy,dual-site fluorescent probe(CNN)for detection of protease with high selectivity was developed.By integrated substrates of nitroreductase and human quinone reductase1 to the cinnamonitrile derivative,a dual-site fluorescent probe(CNN)for simultaneous detection of NRT and hNQO1 was developed.In present of both of NRT and hNQO1,coumarin dye was in situ generated via intramolecular cyclization reaction.With the use of the probe CNN,cancer cell can be distinguished effectively for normal cells.The probe will be a great benefit to improving the accurate diagnosis of cancer.