Studies On The Construction And Recognition Properties Of Fluorescent Probes Based On DCM

Dicyanomethylene-benzopyran?DCM?derivatives whose fluorescence emission wavelength usually exceeds 600 nm are widely used in the fileds of nonlinear optical materials,electroluminescence and so on.Generally,the recognition mechanism is the process of intramolecular charge transfer?ICT?due to its typical"push-pull electron"structure.Different derivatives of DCM as a fluorophore have been applied to study a number of chemical substances in cels,tissues and mice.In this paper,five fluorescent probes named F1-F5 were designed and synthesized,including four DCM-based probes and one benzopyrone-based probe.Spectral properties of each probe were studied and discussed.1.F1 is a probe for relay recognition of Cu²⁺,Cys?cysteine?and GSH?glutathione?,which can detect Cu²⁺,Cys and GSH in the mixture solution of CH₃CN and water?50 mM Tris-HCl buffer,pH=7.4,v:v,8:2?.It not noly belongs to a type of"ON-OFF-ON"probe molecule,but also can be applied to detect Cu²⁺,Cys and GSH in living cels.2.In ethanol solution,the complexation of probes F2,F3 and F4 with Zn²⁺,respectively,leads to rapidly recognizing Zn²⁺.For F2,in the absence of Zn²⁺yellow fluorescence was observed,while in the presence of Zn²⁺green fluorescence was discovered.For F3 or F4,without Zn²⁺there was little fluorescence,while with Zn²⁺strong red fluorescence was observed.3.F5 can be applied for the detection of Cys in the mixture solution of DMSO and water?10 mM PBS buffer,pH=7.4,v:v,8:2?.The recognition process takes a little time,and the process of detecting Cys is via the substitution and cyclization reaction with chloroacetate to generate the fluorescent substance.