Screening And Identification Of Transformation Rare Ginsenoside And Optimization Of Transforming Conditions From Cordyceps Fungus In Changbai Mountain

Panax ginseng C.A.Meyer is A famous traditional Chinese medicine plan.Gingenosides are the main pharmacological active component of ginseng,the Component content and pharmacological action of single ginsenoside are different.Some rare ginsenosides exhibited various biological and pharmacological activities,including anti-tumor,anti-hyperglycemic,and anti-aging effects.The preparation of rare ginseng saponins is becoming a hot spot of current research.This research mainly by microbial conversion to Changbai mountain of wild cordyceps fungi strains of total saponins of ginseng coarse extraction by liquid fermentation,and in the process of transformation of?-D-glycosidase enzymes activity test,optimized condition,verify the transformation path,provide some ideas to the late industrialization,improve the reasonable utilization of ginseng.1.Ginseng total saponins were separated from ginseng by uesing Microwave and cellulase,These substrates include ginsenoside Re,Rb₁,Rg₁,Rd,etc2.5?-D-glucosidase producing strains was screened from Cordyceps Fungus by?-D-glucosidase enzyme production experimen.strain ZZ0501.sp had the strongest enzyme production capacity.3.Optimize the strains enzyme production conditions,response surface method was used to optimize single factor experimental conditions,experimental results demonstrated that cultivation medium composition was as follows:wheat bran 1%,peptone0.3%,22?,initial pH 7.0,cultured120 hours,the?-D-glucosidase activity of the strains under the optimized fermentation conditions are from 5.56U/mL to 36.76U/mL,increased the enzyme activity by 661%compared with that in the basic medium,achieving the purpose of improving the enzyme activity of?-D-glucosidase..4.The transformation path was qualitatively analyzed by TLC,and the transformation result was quantitatively detected by HPLC.Finally,The best condition of HPLC was attained as follows:columnPgrandsilSTCC18?4.6mmx150mm,5?m?;Mobile acetonitrile?A?:water?B?;gradient elution;Column temperature 30?;wavelength:203nm;1.0mL/min;Injection volume:5?L.In the detection of monomer saponins fermentation,it was known that the transformation path of the five strains was:Rg₁?Rh₁;Rb₁?Rd,Rb₁?Rd?Rg₃.5.Molecular biology and morphological identification were performed on 5 strains with transformationability,andtheresultswereasfollows:ZZ0501.spisCordyceps fumosorosea;QT0501.sp is Isaria fumosorosea;SM0501.sp is Isaria fumosorosea;YP0501.sp is Isaria farinosa;Strain Y30501.sp is Cordyceps militaris.