Preparation Of CeO₂ Catalytic Material And Its Application In Prostate Cancer Detection

Prostate cancer?PCa?is the most commonly diagnosed disease in men and the second most common cancer in the world.At present,the most commonly used prostate cancer detection methods are:digital rectal examination?DRE?,rectal ultrasound?TRUS?,biopsy and PSA blood test.These methods all need to go to the hospital,and have some pain and long time.Therefore,it is urgent to explore a simple new technology for prostate cancer detection.In this paper,three different morphologies of cerium oxide materials?flower-like CeO₂,hollow spherical CeO₂,shuttle-like CeO₂?were prepared and characterized?scanning electron microscopy,X-ray diffraction,Raman spectroscopy?;The preparation method of the disc-shaped CeO₂ was analyzed and optimized to determine the optimal preparation conditions:1 g cesium chloride?CeCl₃?7H₂O?,1.0 g urea,15 mL deionized water,0.3 g PVP,0.6 mL glacial acetic acid,calcination The temperature was 500°C and the calcination time was 4 h.The simulated enzyme activity of the material was verified by TMB chromogenic reagent.The simulated enzyme activity of three sulfhydryl materials was combined with the specificity of DNA aptamer to construct a simple formula.The prostate cancer detection technology is optimized for the detection technology.When the reaction temperature is 30°C,the TMB dosage is 3.5 mL,the DNA dosage is 6 OD,the color development time is 60 min,and the adsorption time is20 min.At the time,the semi-quantitative analysis of the prostate cancer marker PSA?the concentration of PSA salt solution was 4.0 ng/mL to 10.0 ng/mL?was achieved by using the disk-shaped ceria and DNA aptamer construction detection technology.Explore and develop a test strip for the detection of prostate cancer by cerium oxide,and achieve semi-quantitative determination of the PSA concentration in the simulated blood.