Research And Development Of Domestic Enzyme Machine And Study On Mulberry Fermentation Technology

The jiaosu industry in China started late,and the market potential of enzyme-related products needs to be developed.Market research shows that there are many problems in the existing domestic brewing equipment,such as lack of safety,single function,easy to infect bacteria and difficult to control the quality of the finished product.In order to solve the above problems,a domestic enzyme machine is developed in this paper.The practicability and fermentation process parameters of domestic enzyme machine were verified.The main contents of this paper are as follows:(1)Based on the principle of bioengineering equipment,the functional system and structure of domestic enzyme machine are designed:safety system,heating system,cooling system and control system are constructed;The overall structure is composed of sealing cover,fermentation tank and supporting pedestal.The mechanical design size of enzyme machine is determined by using standard elliptical head and 20L fermentation volume as the design goal,and the prototype is produced by processing plant.(2)Three strains suitable for enzyme fermentation were obtained by domestication scheme.The order of activity of the strains was as follows:Lactobacillus plantarum(XN-Z)>Lactobacillus acidophilus(XN-S)>Lactobacillus German(XN-D).The optimum addition ratio of 46%:32.3%:21.7%,sensory evaluation 81,and the number of living bacteria 6.63×10~8 cfu/mL.(3)Through single factor experiment,the optimum fermentation conditions were obtained as follows:fermentation temperature 31-35?,strain addition 4-6%,total fermentation time 100-130h.The orthogonal test was carried out with pH value and free radical scavenging rate as indexes.The fermentation time was 115h,the fermentation temperature was 33?,and the strain addition rate was 5%.The pH value was 3.22,the taste was suitable,and the free radical scavenging rate reached 83%.(4)The fermentation process parameters were applied to the actual production of domestic enzyme machine,and the free amino acids,DPPH free radical scavenging ability and anthocyanin content of domestic enzyme finished product and commercial enzyme machine fermentation product and market finished product were compared.The total content of free amino acids was determined by Hitachi L-8900 amino acid analyzer.It was found that 16 kinds of free amino acids.Enzyme machine fermentation products>market enzyme machine fermentation products>market products.The total amount was 1244.5 mg/L,which was 2 times higher than that of other products.(5)The DPPH free radical scavenging ability of enzymes at different dilution ratios was measured by ultraviolet spectrophotometer with vitamin C as reference.The IC50=8.94 of domestic enzyme machine products was equivalent to vitamin C of22.5mg/L.The scavenging rate of free radicals was the strongest.Proanthocyanidins were determined by high performance liquid chromatography(HPLC).The results showed that enzyme machine fermentation products>market enzyme machine fermentation products>market products,enzyme machine fermentation products was6.12 mg/L,The pH value of other indexes of domestic enzyme machine products is 3.09.Through comparison,it is found that the domestic enzyme machine has good practicability.And the fermentation process parameters are scientific,the quality of fermentation products is better.